Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB4850_RS30400 Genome accession   NZ_CP163163
Coordinates   3470868..3472382 (+) Length   504 a.a.
NCBI ID   WP_414443113.1    Uniprot ID   -
Organism   Burkholderia sp. 22PA0106     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3465868..3477382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4850_RS30375 (AB4850_30360) kdsD 3467066..3468049 (+) 984 WP_414443108.1 arabinose 5-phosphate isomerase KdsD -
  AB4850_RS30380 (AB4850_30365) - 3468049..3468588 (+) 540 WP_414443109.1 KdsC family phosphatase -
  AB4850_RS30385 (AB4850_30370) lptC 3468592..3469206 (+) 615 WP_414443110.1 LPS export ABC transporter periplasmic protein LptC -
  AB4850_RS30390 (AB4850_30375) lptA 3469225..3469896 (+) 672 WP_414443111.1 lipopolysaccharide transport periplasmic protein LptA -
  AB4850_RS30395 (AB4850_30380) lptB 3469893..3470669 (+) 777 WP_414443112.1 LPS export ABC transporter ATP-binding protein -
  AB4850_RS30400 (AB4850_30385) rpoN/rpoN1 3470868..3472382 (+) 1515 WP_414443113.1 RNA polymerase factor sigma-54 Machinery gene
  AB4850_RS30405 (AB4850_30390) hpf 3472536..3472901 (+) 366 WP_414443114.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AB4850_RS30410 (AB4850_30395) - 3473028..3473584 (+) 557 Protein_3041 PTS sugar transporter subunit IIA -
  AB4850_RS30415 (AB4850_30400) hprK 3473713..3474681 (+) 969 WP_013699410.1 HPr(Ser) kinase/phosphatase -
  AB4850_RS30420 (AB4850_30405) rapZ 3474755..3475648 (+) 894 WP_414443115.1 RNase adapter RapZ -
  AB4850_RS30425 (AB4850_30410) - 3475683..3476318 (+) 636 WP_414445659.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 504 a.a.        Molecular weight: 55523.42 Da        Isoelectric Point: 4.9483

>NTDB_id=1028964 AB4850_RS30400 WP_414443113.1 3470868..3472382(+) (rpoN/rpoN1) [Burkholderia sp. 22PA0106]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAIAQNPLLENDEWLASPLRVGADGTLIPQAPPSSAPEPMHG
NGSSSSSSSDSSSERSDRDERNGDDYDSYGSDGGDSSQWNLEDYGRSSSASDDDDLPPLQVQEATTSLREHLSSQLRVTQ
AGPRDRALVMFLIESLDDDGYLGAALDEVMTDLPEELEVDVDELGAALALLHSFDPAGVGARSASECLRLQLLRLDPSPT
RSLSLDIVSQHLELLAARDFTRLRKHLKANDDELRDAHALIRSLEPFPGAAYGKSEADYVVPDIMVRKSGQNWIAELNPE
VVPKLRINHLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFTHGEIAMRPLVLREIAD
TLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDQKSPLSDSRIAELLAEQGFV
VARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1515 bp        

>NTDB_id=1028964 AB4850_RS30400 WP_414443113.1 3470868..3472382(+) (rpoN/rpoN1) [Burkholderia sp. 22PA0106]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCACCTCGCGCTTACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCATCGCCCAGAACCCGCTGCTCGAGAACGACGAGTGGCTCG
CGAGCCCGCTGCGCGTGGGTGCCGACGGCACGCTGATCCCGCAGGCGCCGCCGTCCTCGGCACCCGAGCCGATGCACGGC
AACGGCTCGTCGAGCAGCAGCAGTTCGGATTCATCGAGCGAGCGATCGGACCGCGACGAGCGTAACGGCGACGATTACGA
CTCCTATGGATCCGACGGCGGCGATTCGAGCCAGTGGAACCTGGAAGATTACGGCCGCTCGTCGAGCGCCTCCGATGACG
ACGATCTCCCGCCGCTGCAGGTGCAGGAAGCCACGACCTCGCTGCGCGAGCATCTTTCGTCGCAGTTGCGCGTCACGCAG
GCCGGCCCGCGCGACCGCGCGCTGGTCATGTTCCTGATCGAATCGCTGGACGACGACGGCTATCTCGGCGCGGCGCTCGA
CGAGGTCATGACCGACCTGCCCGAAGAACTCGAAGTGGATGTGGACGAACTGGGCGCCGCGCTCGCACTGCTGCACAGCT
TCGATCCGGCCGGCGTCGGCGCGCGCTCGGCATCCGAATGCCTGCGCCTGCAACTGCTGCGGCTCGATCCGTCCCCCACG
CGCTCGCTGTCGCTCGACATCGTCTCGCAGCATCTCGAACTGCTGGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCT
GAAGGCCAACGACGACGAGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTCGAACCGTTCCCGGGCGCCGCCTACGGCA
AGTCTGAAGCCGACTACGTGGTGCCCGACATCATGGTCCGCAAGAGCGGCCAGAACTGGATCGCGGAGCTCAATCCGGAG
GTGGTGCCGAAGCTGCGCATCAATCATCTGTACGCCAACATCCTGCGCAACAGCCGAGGCGATCCGAGCACCGGATCGCT
GAAGCAGCAGCTCCAGGAAGCACGCTGGCTGATCAAGAATATCCAGCAGCGTTTCGAGACGATTTTGCGTGTCGCGCAGG
CCATCGTGGAGCGTCAGAAGAACTTTTTTACGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGAT
ACGCTGGGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAATACATGCTGACCCCGTTCGGTACTCTTGAATT
TAAGTACTTCTTCGGGTCGCATGTTTCGACAGACACGGGGGGCGCTGCCTCTTCCACGGCAATCCGCGCCCTCATCAAGC
AACTGATAGGAGCCGAAGACCAGAAATCTCCTCTATCGGACAGCCGAATAGCCGAACTGCTGGCGGAACAAGGGTTCGTG
GTAGCGCGTCGTACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTCAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(347-502)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-331)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.928

100

0.679

Multiple sequence alignment