Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   LLA12_RS03290 Genome accession   NZ_LT599049
Coordinates   654369..656597 (+) Length   742 a.a.
NCBI ID   WP_021723124.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain A12     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 649369..661597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLA12_RS03255 (LLA12_00682) - 649858..650526 (+) 669 WP_021723126.1 hypothetical protein -
  LLA12_RS03260 (LLA12_00683) - 650697..651593 (+) 897 WP_003129543.1 SPFH domain-containing protein -
  LLA12_RS03270 (LLA12_00685) - 651968..652228 (+) 261 WP_003129544.1 SemiSWEET family transporter -
  LLA12_RS03280 (LLA12_00687) rnz 652585..653508 (+) 924 WP_003129545.1 ribonuclease Z -
  LLA12_RS03285 (LLA12_00688) - 653501..654202 (+) 702 WP_021723125.1 SDR family NAD(P)-dependent oxidoreductase -
  LLA12_RS03290 (LLA12_00689) recJ 654369..656597 (+) 2229 WP_021723124.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  LLA12_RS03295 (LLA12_00690) - 656720..657232 (+) 513 WP_012897343.1 adenine phosphoribosyltransferase -
  LLA12_RS03300 (LLA12_00691) rpoE 657450..658013 (+) 564 WP_012897344.1 DNA-directed RNA polymerase subunit delta -
  LLA12_RS03305 (LLA12_00692) mltG 658150..659802 (+) 1653 WP_021723123.1 endolytic transglycosylase MltG -
  LLA12_RS03310 (LLA12_00693) greA 659862..660335 (+) 474 WP_025016857.1 transcription elongation factor GreA -
  LLA12_RS03315 (LLA12_00694) - 660512..660967 (+) 456 WP_010905471.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 742 a.a.        Molecular weight: 83512.65 Da        Isoelectric Point: 4.7766

>NTDB_id=1028873 LLA12_RS03290 WP_021723124.1 654369..656597(+) (recJ) [Lactococcus lactis subsp. lactis strain A12]
MIKAKYDWKVADTAISEDFLKIAKKHKLDELTSRVLYQRGIHAEAEIEQFLKPSLENLHDPFLLHDMEKATGRILSAIEQ
GENILIYGDYDADGMTASSVMKSALDELGAEAQVYLPNRFTDGYGPNLDVYQYYIKNENINLIITVDNGVAGLEAITWAQ
ENGVDVIVTDHHSIPDQLPPAYAIVHPGHPDSQYPFKYLAGVGVAFKVACALLEYAPSEMLDLVAIGTIADMVSLTDENR
ILVAHGLKVLAQTERAGLQELMKYAGVDFDKITEETVGFQIAPRLNALGRLDDPNPAIELLTGWDEDEAHEIAKMIDQKN
SERKEIVEKIHNEALSMLTDEPVQILYHKDWHKGVLGIVAGRLLEAIHKPVIMLAQEDGILRGSARSIENFDIFKALNAH
RELFIAFGGHKQAAGMTLSLENVEAVKKAMIDYIVDNHLDMSKKSPLEIADRCHLDDISLSTITNLAKLAPFGMDNPKPR
FLIEDYKVIQSRSMGKDNAHLKLKIQEEKQQIDAVYFQHGSEELEFEQAQTKLVATLSSNSWNGNTSLQLMIEDADSVGV
ELLDIRSKQIPIPKEANIFSQNQLKHGIMEDVLVIEETPGDLTGLSVLKEAVSKAASDDFKMIYFKNKITDSYYLTGSGT
REEFARLYKAIYQFPEFDIRYKLKSLADYLKIPHLLLVKMIKIFEELEFVSIDNGLMTVNKTVDKREISESTIYQELEKI
VKMQELFALAPVKEIYQNLLEK

Nucleotide


Download         Length: 2229 bp        

>NTDB_id=1028873 LLA12_RS03290 WP_021723124.1 654369..656597(+) (recJ) [Lactococcus lactis subsp. lactis strain A12]
ATGATAAAAGCAAAATATGATTGGAAAGTGGCTGATACAGCCATTTCCGAAGATTTTCTAAAGATAGCGAAAAAACATAA
ATTAGATGAATTGACAAGTCGCGTGCTTTATCAAAGAGGAATTCATGCAGAAGCTGAAATTGAGCAATTTTTAAAGCCAA
GTTTAGAAAATTTACATGACCCTTTTCTACTTCATGATATGGAAAAAGCAACAGGGCGTATTTTATCAGCAATTGAGCAA
GGTGAAAACATCTTAATTTATGGTGACTATGATGCTGACGGAATGACTGCATCATCAGTCATGAAATCCGCTCTGGATGA
ATTAGGAGCTGAGGCTCAAGTTTATTTACCCAATCGTTTTACTGACGGTTACGGGCCAAATCTTGACGTTTATCAGTACT
ATATTAAGAATGAAAATATCAATCTTATTATTACTGTAGATAATGGGGTTGCTGGCTTGGAGGCAATTACCTGGGCACAG
GAAAATGGAGTGGATGTAATTGTTACTGACCATCACTCAATTCCTGACCAACTTCCCCCTGCTTATGCCATTGTCCACCC
CGGACATCCTGACAGTCAATATCCTTTCAAATATTTGGCAGGAGTTGGTGTCGCCTTCAAAGTTGCCTGTGCTCTTTTAG
AATATGCACCCAGTGAAATGCTTGATTTAGTTGCAATTGGTACAATTGCTGACATGGTCAGTTTGACTGATGAAAACCGA
ATTTTAGTGGCTCATGGTCTAAAAGTTTTGGCCCAAACAGAGCGTGCCGGCCTACAAGAATTAATGAAGTATGCTGGGGT
TGATTTTGATAAAATCACTGAGGAAACGGTTGGTTTTCAAATTGCTCCTCGTTTAAACGCTTTGGGACGTCTCGATGACC
CAAACCCAGCCATTGAATTGCTGACAGGTTGGGATGAAGATGAAGCGCATGAAATTGCCAAAATGATTGACCAAAAAAAT
TCAGAGCGAAAAGAAATTGTGGAAAAAATTCACAATGAAGCTCTGAGTATGCTGACAGATGAACCTGTTCAGATTTTATA
TCATAAGGACTGGCATAAAGGTGTCTTAGGAATTGTTGCGGGTCGCTTGTTAGAAGCCATACATAAGCCAGTAATCATGC
TGGCCCAAGAAGATGGGATTTTGCGTGGGTCTGCTCGGTCAATCGAAAATTTTGATATTTTCAAGGCATTAAATGCCCAT
CGTGAACTTTTTATTGCTTTTGGTGGTCATAAACAAGCCGCAGGAATGACTTTAAGTCTGGAAAACGTAGAAGCAGTTAA
AAAGGCAATGATTGATTATATCGTGGACAATCATTTAGATATGTCTAAAAAAAGTCCCTTAGAAATTGCTGACCGTTGCC
ACTTAGACGATATTTCGCTCTCAACAATCACTAATTTAGCAAAATTGGCCCCCTTTGGCATGGATAATCCTAAGCCTCGA
TTTTTAATCGAAGATTATAAGGTGATTCAAAGCCGAAGTATGGGGAAAGACAATGCTCATCTTAAATTAAAAATTCAGGA
AGAAAAGCAACAAATTGATGCCGTTTATTTCCAACATGGTTCAGAAGAATTGGAATTCGAACAGGCACAAACAAAATTAG
TCGCAACACTTTCAAGTAATAGTTGGAATGGAAATACGAGTCTTCAATTAATGATTGAAGATGCTGATTCAGTTGGTGTC
GAACTTCTTGATATAAGAAGTAAACAAATTCCTATCCCAAAAGAGGCCAATATTTTTTCTCAAAATCAGCTAAAACATGG
TATAATGGAAGATGTGCTTGTCATCGAAGAGACACCTGGAGATTTAACAGGTTTGTCAGTATTAAAAGAAGCTGTCAGTA
AAGCAGCGTCGGATGATTTTAAAATGATTTATTTTAAAAATAAAATCACTGATAGCTATTATTTAACAGGAAGTGGAACA
CGAGAAGAATTTGCAAGACTTTATAAAGCAATTTATCAATTTCCAGAATTTGATATTCGCTATAAATTAAAATCACTTGC
AGATTATTTGAAAATCCCTCATCTCTTGCTTGTCAAGATGATAAAAATCTTTGAGGAACTTGAATTTGTCAGCATTGACA
ATGGCTTAATGACCGTTAATAAGACGGTCGATAAACGCGAAATTTCTGAGAGTACAATTTATCAAGAACTAGAAAAAATT
GTAAAAATGCAAGAACTTTTTGCTTTAGCACCCGTCAAAGAAATTTATCAAAATTTATTAGAAAAATAA

Domains

Predicted by InterProScan.

RecJ OB domain (RecJ_OB)

(454-554)

DHHA1 domain (DHHA1)

(317-434)

DHH family (DHH)

(83-228)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(608-737)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

56.779

100

0.57
  recJ Bacillus subtilis subsp. subtilis str. 168

36.457

100

0.383