Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB4O75_RS20275 Genome accession   NZ_CP163109
Coordinates   4211708..4213141 (+) Length   477 a.a.
NCBI ID   WP_038416793.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain W55 isolate China:Kunming     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4206708..4218141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4O75_RS20245 (AB4O75_20245) - 4207275..4208252 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  AB4O75_RS20250 (AB4O75_20250) kdsD 4208266..4209252 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  AB4O75_RS20255 (AB4O75_20255) kdsC 4209267..4209833 (+) 567 WP_017382729.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AB4O75_RS20260 (AB4O75_20260) lptC 4209830..4210405 (+) 576 WP_022649529.1 LPS export ABC transporter periplasmic protein LptC -
  AB4O75_RS20265 (AB4O75_20265) lptA 4210374..4210928 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AB4O75_RS20270 (AB4O75_20270) lptB 4210935..4211660 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  AB4O75_RS20275 (AB4O75_20275) rpoN/rpoN1 4211708..4213141 (+) 1434 WP_038416793.1 RNA polymerase factor sigma-54 Machinery gene
  AB4O75_RS20280 (AB4O75_20280) hpf 4213164..4213451 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  AB4O75_RS20285 (AB4O75_20285) ptsN 4213534..4214025 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AB4O75_RS20290 (AB4O75_20290) rapZ 4214071..4214925 (+) 855 WP_003861870.1 RNase adapter RapZ -
  AB4O75_RS20295 (AB4O75_20295) npr 4214922..4215194 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  AB4O75_RS20300 (AB4O75_20300) mtgA 4215234..4215959 (-) 726 WP_023298721.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AB4O75_RS20305 (AB4O75_20305) elbB 4215956..4216609 (-) 654 WP_038416794.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54142.04 Da        Isoelectric Point: 4.3409

>NTDB_id=1028820 AB4O75_RS20275 WP_038416793.1 4211708..4213141(+) (rpoN/rpoN1) [Enterobacter hormaechei strain W55 isolate China:Kunming]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEIDAQQTQDTETLDSVDALEQKEMPE
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISEHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=1028820 AB4O75_RS20275 WP_038416793.1 4211708..4213141(+) (rpoN/rpoN1) [Enterobacter hormaechei strain W55 isolate China:Kunming]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAACAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGATAGACGCTCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAG
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGATTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGAGCATCTGGATTTGCTGGCAAATCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTCAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGTCCGGGGCAATCTATCCAGACCAG
CGAACCGGAATACGTTATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAAAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGCCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment