Detailed information NTG1028481

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AB4O93_RS20705
• Genome accession: NZ_CP163017
• Coordinates: 4212724..4213524 (+)
• Length: 266 a.a.
• NCBI ID: WP_023292017.1
• Uniprot ID: A0A483KIE1
• Organism: Klebsiella quasipneumoniae strain W183
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4207724..4218524

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB4O93_RS20670 (AB4O93_20670)	gspH	4207877..4208392 (+)	516	WP_064158221.1	type II secretion system minor pseudopilin GspH	-
AB4O93_RS20675 (AB4O93_20675)	gspI	4208389..4208754 (+)	366	WP_032452553.1	type II secretion system minor pseudopilin GspI	-
AB4O93_RS20680 (AB4O93_20680)	gspJ	4208751..4209347 (+)	597	WP_044521061.1	type II secretion system minor pseudopilin GspJ	-
AB4O93_RS20685 (AB4O93_20685)	gspK	4209344..4210324 (+)	981	WP_077266698.1	type II secretion system minor pseudopilin GspK	-
AB4O93_RS20690 (AB4O93_20690)	gspL	4210317..4211513 (+)	1197	WP_044521060.1	type II secretion system protein GspL	-
AB4O93_RS20695 (AB4O93_20695)	-	4211506..4211991 (+)	486	WP_064158220.1	type II secretion system protein M	-
AB4O93_RS20700 (AB4O93_20700)	-	4211981..4212709 (+)	729	WP_064158219.1	type II secretion system protein N	-
AB4O93_RS20705 (AB4O93_20705)	pilD	4212724..4213524 (+)	801	WP_023292017.1	A24 family peptidase	Machinery gene
AB4O93_RS20710 (AB4O93_20710)	hrpB	4213572..4216001 (-)	2430	WP_368989060.1	ATP-dependent helicase HrpB	-
AB4O93_RS20715 (AB4O93_20715)	thpR	4216074..4216610 (+)	537	WP_023292015.1	RNA 2',3'-cyclic phosphodiesterase	-
AB4O93_RS20720 (AB4O93_20720)	sfsA	4216610..4217326 (+)	717	WP_023292014.1	DNA/RNA nuclease SfsA	-
AB4O93_RS20725 (AB4O93_20725)	dksA	4217489..4217944 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29274.82 Da
• Isoelectric Point: 8.8894

>NTDB_id=1028481 AB4O93_RS20705 WP_023292017.1 4212724..4213524(+) (pilD) [Klebsiella quasipneumoniae strain W183]
MTTLAALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDEPITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQARISWSYPLTELATGLLFILAGALLAPGLALAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQALPQVLLLASASGLAWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=1028481 AB4O93_RS20705 WP_023292017.1 4212724..4213524(+) (pilD) [Klebsiella quasipneumoniae strain W183]
ATGACGACATTAGCCGCATTATCGCTGCATTTTCCCCTTGTCTGGTATGGTTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCGCGCATGCTGACCCAAACCGCGGACGACGAGCCGATAACCCTCAGCA
CGCCGGGCTCATCCTGCCCGCAGTGTCGCCAGCCGATAGCCTGGCGGGACAATATCCCGCTGCTCAGCTTCCTCTGGCTA
GGCCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTTAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCCCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGCTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCAGCGGGCTGGCGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

3D structure

• AlphaFold DB: A0A483KIE1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425