Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AB4073_RS16660 Genome accession   NZ_CP162643
Coordinates   3536241..3537041 (-) Length   266 a.a.
NCBI ID   WP_200043288.1    Uniprot ID   -
Organism   Acinetobacter lactucae strain 128-92     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3531241..3542041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4073_RS16640 (AB4073_16640) - 3532303..3533001 (-) 699 WP_368678082.1 YfgM family protein -
  AB4073_RS16645 (AB4073_16645) hisS 3533030..3534322 (-) 1293 WP_062849551.1 histidine--tRNA ligase -
  AB4073_RS16650 (AB4073_16650) ispG 3534319..3535434 (-) 1116 WP_016144301.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AB4073_RS16655 (AB4073_16655) - 3535456..3536250 (-) 795 WP_200043290.1 DUF4115 domain-containing protein -
  AB4073_RS16660 (AB4073_16660) pilF 3536241..3537041 (-) 801 WP_200043288.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AB4073_RS16665 (AB4073_16665) rlmN 3537056..3538291 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AB4073_RS16670 (AB4073_16670) ndk 3538415..3538846 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  AB4073_RS16675 (AB4073_16675) iscX 3538960..3539160 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  AB4073_RS16680 (AB4073_16680) - 3539260..3539811 (-) 552 WP_016144298.1 phosphatase PAP2 family protein -
  AB4073_RS16685 (AB4073_16685) - 3539836..3541122 (-) 1287 WP_080648637.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29983.14 Da        Isoelectric Point: 9.0424

>NTDB_id=1027772 AB4073_RS16660 WP_200043288.1 3536241..3537041(-) (pilF) [Acinetobacter lactucae strain 128-92]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKTNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMGRYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
VNAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVHTVGQKNQGARALWIGVRVARANADKMGTQALVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1027772 AB4073_RS16660 WP_200043288.1 3536241..3537041(-) (pilF) [Acinetobacter lactucae strain 128-92]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCGGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCGGAAAAAGCAGTAAAAGTGCGTACACAACTTGCTGCTGAACATATCCGTTCTGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAGGCTTTAAGTGTAGATAGCCGCGATGCAACAGCAAACATGATGATGGGGATTTTG
CTACAGCAAGAAGGTAGCAAAACCAACTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATACCAAATGGGGCGTTATAATGATGCGATCGAGCAATTTCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GTGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGTGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCATACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAACGCAAGCATTAGTAAACCAA
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-205)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

92.481

100

0.925
  pilF Acinetobacter baylyi ADP1

75

97.744

0.733

Multiple sequence alignment