Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AB3U43_RS25310 Genome accession   NZ_CP162629
Coordinates   4089887..4090930 (-) Length   347 a.a.
NCBI ID   WP_001013182.1    Uniprot ID   -
Organism   Bacillus cereus strain L6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4084887..4095930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3U43_RS25265 (AB3U43_25265) - 4085318..4085518 (-) 201 WP_023523009.1 YqzE family protein -
  AB3U43_RS25270 (AB3U43_25270) - 4085557..4086054 (-) 498 WP_044795907.1 shikimate kinase -
  AB3U43_RS25275 (AB3U43_25275) - 4086170..4086820 (-) 651 WP_000183860.1 2OG-Fe(II) oxygenase -
  AB3U43_RS25280 (AB3U43_25280) comGG 4086997..4087368 (-) 372 WP_016118625.1 competence type IV pilus minor pilin ComGG -
  AB3U43_RS25285 (AB3U43_25285) comGF 4087365..4087835 (-) 471 WP_000923099.1 competence type IV pilus minor pilin ComGF -
  AB3U43_RS25290 (AB3U43_25290) - 4087805..4088107 (-) 303 WP_000829473.1 hypothetical protein -
  AB3U43_RS25295 (AB3U43_25295) comGD 4088100..4088555 (-) 456 WP_000813143.1 competence type IV pilus minor pilin ComGD -
  AB3U43_RS25300 (AB3U43_25300) comGC 4088552..4088851 (-) 300 WP_001178683.1 competence type IV pilus major pilin ComGC -
  AB3U43_RS25305 (AB3U43_25305) comGB 4088863..4089894 (-) 1032 WP_137070173.1 competence type IV pilus assembly protein ComGB -
  AB3U43_RS25310 (AB3U43_25310) comGA 4089887..4090930 (-) 1044 WP_001013182.1 competence type IV pilus ATPase ComGA Machinery gene
  AB3U43_RS25315 (AB3U43_25315) - 4091136..4091831 (+) 696 WP_000434125.1 metalloregulator ArsR/SmtB family transcription factor -
  AB3U43_RS25320 (AB3U43_25320) - 4091957..4092199 (+) 243 WP_000440721.1 DUF2626 domain-containing protein -
  AB3U43_RS25325 (AB3U43_25325) - 4092306..4093700 (+) 1395 WP_001094336.1 L-cystine transporter -
  AB3U43_RS25330 (AB3U43_25330) - 4093780..4094172 (-) 393 Protein_4101 hypothetical protein -
  AB3U43_RS25335 (AB3U43_25335) - 4094260..4094475 (-) 216 WP_001008326.1 DUF3912 family protein -
  AB3U43_RS25340 (AB3U43_25340) - 4094728..4095039 (+) 312 WP_001093237.1 hypothetical protein -
  AB3U43_RS25345 (AB3U43_25345) - 4095074..4095562 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39185.54 Da        Isoelectric Point: 8.5491

>NTDB_id=1027630 AB3U43_RS25310 WP_001013182.1 4089887..4090930(-) (comGA) [Bacillus cereus strain L6]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRRKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGHELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1027630 AB3U43_RS25310 WP_001013182.1 4089887..4090930(-) (comGA) [Bacillus cereus strain L6]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAGGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCAGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GATATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAAGTA
ATTAGAAGAAAGAAAACACGTCGCATCATTACACTTGAAGATCCTGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGGGATGAGGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATCGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTACTATATGGACATGAGTTAAAACAAGCACTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.62

100

0.556

Multiple sequence alignment