Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   AB3471_RS02035 Genome accession   NZ_CP162520
Coordinates   433851..436070 (-) Length   739 a.a.
NCBI ID   WP_004414620.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. atrofaciens strain UPB 463     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 428851..441070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3471_RS02015 (AB3471_02005) hslU 428971..430308 (-) 1338 WP_003316641.1 ATP-dependent protease ATPase subunit HslU -
  AB3471_RS02020 (AB3471_02010) hslV 430375..430905 (-) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  AB3471_RS02025 (AB3471_02015) - 431320..432015 (-) 696 WP_004414617.1 SPOR domain-containing protein -
  AB3471_RS02030 (AB3471_02020) argS 432017..433753 (-) 1737 WP_003422551.1 arginine--tRNA ligase -
  AB3471_RS02035 (AB3471_02025) priA 433851..436070 (-) 2220 WP_004414620.1 primosomal protein N' Machinery gene
  AB3471_RS02040 (AB3471_02030) rpmE 436240..436461 (+) 222 WP_002551648.1 50S ribosomal protein L31 -
  AB3471_RS02045 (AB3471_02035) - 436475..437278 (+) 804 WP_004414622.1 thermonuclease family protein -
  AB3471_RS02050 (AB3471_02040) - 437461..438729 (+) 1269 WP_003316648.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81853.14 Da        Isoelectric Point: 8.4231

>NTDB_id=1026869 AB3471_RS02035 WP_004414620.1 433851..436070(-) (priA) [Pseudomonas syringae pv. atrofaciens strain UPB 463]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNKDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPLKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSAGLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=1026869 AB3471_RS02035 WP_004414620.1 433851..436070(-) (priA) [Pseudomonas syringae pv. atrofaciens strain UPB 463]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCAGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGCCGGGAAATGATCGGCATTCTGGTAGAAGTGG
TCGATCACAGCGAAGTGCCCGCCGACAAGCTCAAGCCGGCGATCGCCCTGCTCGACAGCGAAGCGCCGCTGCCACCTGCG
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTGCT
GTTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAGGAACGCTTCTGGCACGTGGCCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCGCCCAAGCAGCGCGAGGCCCTGACCACTCTTGCCCAGCACCCGCACGGGGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTTAACAAGGACAGCCTCAACCTGTTGCTGGCCAAGGAACTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCGCTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGGTTCGACAGCTTTCATGCGTTTCTGCTGGCCGGCGTCACCGGCAGCGGCAAGACTGAAGTCTATCTGCAA
CTAATCCGCGAAACCCTCGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAACGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGATGCCTGGC
TGGCAGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGCTGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGCTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCCTTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTTGGCTCGGCCACACCGTCTTTGGAAAGCCTGCACAACGCTTACACCG
GCCGCTACGGCCTGCTGCGCCTGAATGAGCGCGCGGGCGGCGCACAGCAGCCGCGTTTCATGCGCCTGGACGTCAAGAGC
CGACCGCTGGACAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGCCAGACGCTCGCGGCAGGCCAGCAGGTCCTGGT
GTTTCTCAACCGTCGCGGCTTTGCGCCAACCTTGCTGTGCCACGACTGCGGCTGGATGTCCGGTTGCCAACGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAGCTGCGCTGCCATCACTGCGGGTATGTCGAACGCGTACCGCGTCAGTGC
CCGTCGTGCGGCAAAGTGGATTTGCGCCCGGTAGGCGCGGGAACAGAGCGCGCCGAAGAGCGCCTGGCGATTCTGTTTCC
GGACTACCCCGTACTGCGTGTCGACCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATCCAGC
GCGGTCAGCCATGCATTCTGGTCGGCACCCAAATGCTAGCCAAGGGCCATCACTTTCCCCGGGTCACGCTGGTCTCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAATTAATCGTGCAGGTGGCAGG
TCGTGCCGGGCGCGCCGAAGAGCCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCACCCACTGCTGATCCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAGGCCTTGAGCGAGCGCCGTTCGGCGGGGCTACCACCGTTTTCACACCTC
GCACTGCTGCGCGCTGAAGCGCACAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCGGAGCAACT
GGTTGCGCAGATGGCGCTGGGCGGCATCGAGCTGCTGGGCCCGGTTCCGGCACCGATGGAACGCCGCGCCGGGCGTTATC
GTGCGCAACTGCTGGTGCAGTCCAGCGCCCGCGCACCCTTGCATAAATTGCTTGCCACCTGGCTGCTCGCGCTGGAGCAA
ATGCCCAGCGGTCGGCAGGTCAGATGGTCGCTGGATGTGGATCCTGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment