Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   AB3453_RS12950 Genome accession   NZ_CP162420
Coordinates   2560989..2562338 (+) Length   449 a.a.
NCBI ID   WP_000815640.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM22741     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 2555328..2570753 2560989..2562338 within 0


Gene organization within MGE regions


Location: 2555328..2570753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3453_RS12930 (AB3453_12930) - 2555328..2556818 (-) 1491 WP_000554504.1 RNA-binding domain-containing protein -
  AB3453_RS12935 (AB3453_12935) - 2557490..2558539 (-) 1050 WP_000613248.1 hypothetical protein -
  AB3453_RS12940 (AB3453_12940) - 2558678..2558968 (+) 291 WP_001036438.1 cassette chromosome ssDNA-binding protein -
  AB3453_RS12945 (AB3453_12945) - 2558968..2560755 (+) 1788 WP_000122620.1 cassette chromosome replicative helicase -
  AB3453_RS12950 (AB3453_12950) ccrA 2560989..2562338 (+) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  AB3453_RS12955 (AB3453_12955) ccrB 2562360..2563988 (+) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  AB3453_RS12960 (AB3453_12960) - 2564510..2564860 (+) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  AB3453_RS12965 (AB3453_12965) - 2564947..2565258 (+) 312 WP_000700851.1 DUF960 family protein -
  AB3453_RS12970 (AB3453_12970) - 2565273..2565779 (+) 507 WP_001092189.1 DUF1643 domain-containing protein -
  AB3453_RS12975 (AB3453_12975) - 2565831..2567438 (+) 1608 Protein_2514 IS1182 family transposase -
  AB3453_RS12980 (AB3453_12980) - 2567429..2567689 (-) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  AB3453_RS12985 (AB3453_12985) - 2567661..2568647 (-) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  AB3453_RS12990 (AB3453_12990) mecA 2568744..2570753 (+) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52663.34 Da        Isoelectric Point: 9.9639

>NTDB_id=1025177 AB3453_RS12950 WP_000815640.1 2560989..2562338(+) (ccrA) [Staphylococcus aureus strain TUM22741]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHIIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1025177 AB3453_RS12950 WP_000815640.1 2560989..2562338(+) (ccrA) [Staphylococcus aureus strain TUM22741]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACACCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAGCAAACACAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAACACATCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATTGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Recombinase (Recombinase)

(169-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

75.947

100

0.759

Multiple sequence alignment