Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   AB3454_RS05025 Genome accession   NZ_CP162418
Coordinates   969108..970457 (-) Length   449 a.a.
NCBI ID   WP_000815640.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM22750     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 960692..976119 969108..970457 within 0


Gene organization within MGE regions


Location: 960692..976119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3454_RS04985 (AB3454_04985) mecA 960692..962701 (-) 2010 WP_267811135.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  AB3454_RS04990 (AB3454_04990) - 962798..963784 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  AB3454_RS04995 (AB3454_04995) - 963756..964016 (+) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  AB3454_RS05000 (AB3454_05000) - 964007..965615 (-) 1609 Protein_944 IS1182 family transposase -
  AB3454_RS05005 (AB3454_05005) - 965667..966173 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  AB3454_RS05010 (AB3454_05010) - 966188..966499 (-) 312 WP_000700851.1 DUF960 family protein -
  AB3454_RS05015 (AB3454_05015) - 966586..966936 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  AB3454_RS05020 (AB3454_05020) ccrB 967458..969086 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  AB3454_RS05025 (AB3454_05025) ccrA 969108..970457 (-) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  AB3454_RS05030 (AB3454_05030) - 970691..972478 (-) 1788 WP_000122620.1 cassette chromosome replicative helicase -
  AB3454_RS05035 (AB3454_05035) - 972478..972768 (-) 291 WP_001036438.1 cassette chromosome ssDNA-binding protein -
  AB3454_RS05040 (AB3454_05040) - 972908..973957 (+) 1050 WP_340790673.1 ACP synthase -
  AB3454_RS05045 (AB3454_05045) - 974630..976119 (+) 1490 Protein_953 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52663.34 Da        Isoelectric Point: 9.9639

>NTDB_id=1025109 AB3454_RS05025 WP_000815640.1 969108..970457(-) (ccrA) [Staphylococcus aureus strain TUM22750]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHIIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1025109 AB3454_RS05025 WP_000815640.1 969108..970457(-) (ccrA) [Staphylococcus aureus strain TUM22750]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACACCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAGCAAACACAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAACACATCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATTGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Recombinase (Recombinase)

(169-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

75.947

100

0.759

Multiple sequence alignment