Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB2B48_RS15270 Genome accession   NZ_CP162271
Coordinates   3168712..3170145 (+) Length   477 a.a.
NCBI ID   WP_185667794.1    Uniprot ID   -
Organism   Kluyvera sichuanensis strain lhn-g4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3163712..3175145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2B48_RS15240 (AB2B48_15240) - 3164282..3165256 (+) 975 WP_185667798.1 calcium/sodium antiporter -
  AB2B48_RS15245 (AB2B48_15245) kdsD 3165271..3166257 (+) 987 WP_317983845.1 arabinose-5-phosphate isomerase KdsD -
  AB2B48_RS15250 (AB2B48_15250) kdsC 3166273..3166839 (+) 567 WP_333496262.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AB2B48_RS15255 (AB2B48_15255) lptC 3166836..3167411 (+) 576 WP_185667796.1 LPS export ABC transporter periplasmic protein LptC -
  AB2B48_RS15260 (AB2B48_15260) lptA 3167380..3167934 (+) 555 WP_185667795.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AB2B48_RS15265 (AB2B48_15265) lptB 3167941..3168666 (+) 726 WP_035891167.1 LPS export ABC transporter ATP-binding protein -
  AB2B48_RS15270 (AB2B48_15270) rpoN/rpoN1 3168712..3170145 (+) 1434 WP_185667794.1 RNA polymerase factor sigma-54 Machinery gene
  AB2B48_RS15275 (AB2B48_15275) hpf 3170168..3170455 (+) 288 WP_185667793.1 ribosome hibernation promoting factor -
  AB2B48_RS15280 (AB2B48_15280) ptsN 3170511..3170999 (+) 489 WP_220440879.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AB2B48_RS15285 (AB2B48_15285) rapZ 3171033..3171887 (+) 855 WP_035891172.1 RNase adapter RapZ -
  AB2B48_RS15290 (AB2B48_15290) npr 3171884..3172156 (+) 273 WP_185667791.1 PTS phosphocarrier protein NPr -
  AB2B48_RS15295 (AB2B48_15295) mtgA 3172221..3172940 (-) 720 WP_368299563.1 monofunctional biosynthetic peptidoglycan transglycosylase -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53694.59 Da        Isoelectric Point: 4.3129

>NTDB_id=1024757 AB2B48_RS15270 WP_185667794.1 3168712..3170145(+) (rpoN/rpoN1) [Kluyvera sichuanensis strain lhn-g4]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEVETQETVDNETLDSVDALEKPDMPE
ELPLDASWDEIYTAGTPSGTGADYQDDELPVYQGETTQSLQDYLMWQVELTPFTETDRAIATSIVDAVDDTGYLTVTVED
ILEGIGDEEVGLDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSPATPRITEARLIISDHLDLLANHDFRSLMRVTR
LKEDVLKEAVDLIQSLDPRPGQSIQTGEPEYVIPDVLVRKVNDRWVVELNADSIPRLQINQHYAGMCNNARNDADSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFDLGEEYMKPMVLADIAQTVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVSTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=1024757 AB2B48_RS15270 WP_185667794.1 3168712..3170145(+) (rpoN/rpoN1) [Kluyvera sichuanensis strain lhn-g4]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTTGCCATGACGCCACAGCTGCAACAGGCAATCCGTCTGCTGCA
GCTGTCTACGCTGGAACTTCAGCAGGAACTCCAGCAGGCGCTGGAGAGTAACCCCCTGCTTGAGCAAACCGATCTTCACG
AAGAAGTAGAGACTCAGGAAACCGTCGATAACGAAACGCTGGACAGCGTTGATGCGCTGGAAAAACCTGATATGCCCGAA
GAGCTGCCGCTCGACGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGGACCGGCGCTGATTATCAGGACGA
TGAATTACCGGTTTATCAGGGTGAAACCACCCAGTCGCTTCAGGATTATCTGATGTGGCAGGTTGAGCTGACGCCGTTTA
CCGAGACCGACCGGGCTATTGCGACTTCGATCGTCGACGCGGTGGACGACACCGGCTATCTCACCGTCACGGTGGAAGAC
ATTCTGGAAGGGATTGGCGACGAAGAGGTTGGCCTCGATGAAGTGGAAGCCGTGCTCAAGCGGATCCAACGCTTCGATCC
AGTCGGCGTCGCCGCCAAAGATCTCCGCGATTGCCTGCTGATCCAACTTTCTCAGTTCTCCCCCGCTACGCCGCGAATCA
CTGAAGCCCGTCTGATTATCAGCGATCATCTCGATCTGCTCGCCAACCACGATTTCCGTTCACTGATGCGCGTCACGCGG
CTGAAAGAGGACGTGTTGAAAGAGGCGGTGGATCTGATCCAGTCGCTGGATCCGCGCCCGGGTCAGTCGATCCAGACCGG
CGAGCCGGAGTATGTGATCCCAGACGTACTGGTGCGCAAGGTGAACGATCGCTGGGTCGTTGAGCTGAACGCCGACAGCA
TTCCGCGTCTGCAGATCAACCAACACTACGCGGGAATGTGCAACAACGCCCGTAACGACGCCGACAGCCAGTTTATCCGC
AGCAATCTGCAGGACGCGAAATGGCTGATTAAGAGCCTTGAGAGCCGTAACGACACGCTGCTGCGAGTCAGCCGCTGCAT
CGTTGAACAACAGCAGGCCTTTTTCGATCTCGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGACCG
TCGAGATGCACGAGTCCACCATTTCGCGTGTGACCACGCAAAAGTATTTGCATAGCCCACGAGGCATTTTCGAGCTTAAG
TATTTCTTCTCCAGTCACGTGAGTACCGAGGGCGGCGGCGAGGCCTCTTCCACGGCGATTCGCGCGCTGGTTAAGAAATT
AATTGCCGCGGAGAACCCCGCAAAACCACTGAGCGACAGTAAACTGACCACCATGCTCTCTGAACAGGGTATCATGGTGG
CACGGCGTACCGTTGCAAAGTACCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.455

100

0.482

Multiple sequence alignment