Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB3N47_RS19080 Genome accession   NZ_CP162155
Coordinates   4048772..4049752 (-) Length   326 a.a.
NCBI ID   WP_095427933.1    Uniprot ID   -
Organism   Enterobacter cloacae strain STK66-C     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4043772..4054752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3N47_RS19050 (AB3N47_19050) - 4044648..4045145 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  AB3N47_RS19055 (AB3N47_19055) endA 4045240..4045947 (+) 708 WP_095427934.1 deoxyribonuclease I -
  AB3N47_RS19060 (AB3N47_19060) rsmE 4046000..4046731 (+) 732 WP_013098662.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB3N47_RS19065 (AB3N47_19065) gshB 4046751..4047698 (+) 948 WP_023620782.1 glutathione synthase -
  AB3N47_RS19070 (AB3N47_19070) - 4047785..4048345 (+) 561 WP_013098664.1 YqgE/AlgH family protein -
  AB3N47_RS19075 (AB3N47_19075) ruvX 4048345..4048761 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  AB3N47_RS19080 (AB3N47_19080) pilT 4048772..4049752 (-) 981 WP_095427933.1 type IV pilus twitching motility protein PilT Machinery gene
  AB3N47_RS19085 (AB3N47_19085) - 4049770..4050471 (+) 702 WP_023620785.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB3N47_RS19090 (AB3N47_19090) - 4050493..4051059 (+) 567 WP_013098667.1 YggT family protein -
  AB3N47_RS19095 (AB3N47_19095) yggU 4051056..4051343 (+) 288 WP_013098668.1 DUF167 family protein YggU -
  AB3N47_RS19100 (AB3N47_19100) - 4051356..4051949 (+) 594 WP_013098669.1 XTP/dITP diphosphatase -
  AB3N47_RS19105 (AB3N47_19105) hemW 4051942..4053090 (+) 1149 WP_095427932.1 radical SAM family heme chaperone HemW -
  AB3N47_RS19110 (AB3N47_19110) - 4053147..4053509 (+) 363 WP_095427931.1 endonuclease domain-containing protein -
  AB3N47_RS19115 (AB3N47_19115) - 4053577..4054293 (-) 717 WP_023621456.1 DUF2884 domain-containing protein -
  AB3N47_RS19120 (AB3N47_19120) - 4054350..4054676 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35666.68 Da        Isoelectric Point: 6.3038

>NTDB_id=1024348 AB3N47_RS19080 WP_095427933.1 4048772..4049752(-) (pilT) [Enterobacter cloacae strain STK66-C]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRVGRLEPAPFPSPDVDALLKTWLNDEQQGAWWASGQVDFAVTLTGNQRLRA
SAFKQMKGNSITLRLLPRACPQLSALGVPRAIPELLSHDSGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FVYQSERCLIQQREIGLHSPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRIALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1024348 AB3N47_RS19080 WP_095427933.1 4048772..4049752(-) (pilT) [Enterobacter cloacae strain STK66-C]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAGTAGGCCGCCTTGAACCGGCTCCCTTTCCCTCTCCCGATGTGGATGCGTTACTGAAAACGTGGCTCAACG
ATGAGCAGCAGGGGGCATGGTGGGCAAGCGGGCAGGTGGATTTTGCCGTGACCCTTACGGGAAACCAGCGTCTGCGCGCA
AGTGCCTTTAAACAGATGAAGGGCAACTCCATCACGCTACGCCTGTTGCCCCGGGCCTGTCCGCAGCTCTCCGCTTTGGG
TGTTCCCCGGGCTATCCCGGAACTCTTATCCCACGACAGTGGGCTGATACTGGTGACGGGGGCAACCGGCAGCGGCAAAT
CTACCACCCTTGCGGCAATGGTGGATTTTCTTAATCACCAGACGGATGGGCATATTCTGACGCTGGAAGATCCGGTGGAG
TTTGTCTATCAGAGCGAACGTTGCCTGATTCAGCAGCGAGAGATTGGCCTGCACAGTCCCTCATTTGCCGAGGCGCTGCG
CGCTGCGTTGCGTGAAGATCCGGATGTCATCCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CCGCTGAAACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGTGCCTCTCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAGACTGCTTCC
CGACCTGCAGGGTGGGCGCATCGCGCTGTATGAACTGCTGGTGAATACGCCAGCGGCAGCGAACCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGGATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAGCGA
CGGGCGCAGGGGCGGTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilB Haemophilus influenzae 86-028NP

33.989

100

0.371
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment