Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   AB2J53_RS20045 Genome accession   NZ_CP162020
Coordinates   3935065..3937434 (-) Length   789 a.a.
NCBI ID   WP_368297265.1    Uniprot ID   -
Organism   Cytobacillus firmus strain A4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3930065..3942434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2J53_RS20030 (AB2J53_20030) dtd 3931498..3931938 (-) 441 WP_048010352.1 D-aminoacyl-tRNA deacylase -
  AB2J53_RS20035 (AB2J53_20035) - 3931953..3934148 (-) 2196 WP_061791146.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  AB2J53_RS20040 (AB2J53_20040) - 3934562..3935074 (-) 513 WP_048010354.1 adenine phosphoribosyltransferase -
  AB2J53_RS20045 (AB2J53_20045) recJ 3935065..3937434 (-) 2370 WP_368297265.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  AB2J53_RS20050 (AB2J53_20050) - 3937594..3937911 (-) 318 WP_048010356.1 lipopolysaccharide assembly LapA domain-containing protein -
  AB2J53_RS20055 (AB2J53_20055) - 3937936..3938838 (-) 903 WP_222497680.1 cation diffusion facilitator family transporter -
  AB2J53_RS20060 (AB2J53_20060) secDF 3938973..3941237 (-) 2265 WP_197213322.1 protein translocase subunit SecDF -
  AB2J53_RS20065 (AB2J53_20065) - 3941526..3941822 (-) 297 WP_197208182.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 789 a.a.        Molecular weight: 88147.52 Da        Isoelectric Point: 5.0550

>NTDB_id=1023632 AB2J53_RS20045 WP_368297265.1 3935065..3937434(-) (recJ) [Cytobacillus firmus strain A4]
MLHSRTRWIVRQTEEDKVQRLSKELNISPLAASLLINRGMDTVDSARYFLMDNEQEFHDPFLMTDMAIAVSRIKEAIEKQ
EPILIFGDYDADGVTSTSVMVMALKDLGAAVQFYIPNRFTEGYGPNIPAFEHAAEIGIRLIITVDTGISAVNEAKAAKEL
GMDLIITDHHEPGPELPEALAIVHPKHPESRYPFRELAGVGVAFKLAHALYGEMPEHLLEIAAIGTIADLVSLTGENRLI
AKRGIQKLKSTKNTGLNALFKAARIELPSISEETIGFTIGPRLNAAGRLESADPAVDLLLTNDPYEAQAIAEEIEMLNKE
RQSIVNDIAAQAVHEVETNFPISENSVIVVGKEGWNAGVIGIVASKLVEKFYRPAIVLSYDSEKGLAKGSARSIEGFDLF
KNLSECRDILPHFGGHPMAAGMTLSIDSVDDLRSRLNTLANEQLSEEDFIPVTSIDGVFHLKDINLSSIAEMQLLAPFGM
GNAKPKVMIKDANIAAMRKIGSEQNHIKVTIENDGSSLDGIGFGLGHLHEHISPYSKLSVIGELSINEWNNIKKPQIFLQ
DMAVNSWQLFDIRSLKRLERLQSVIPEANTTWLFFNEEYISKYKPFAGENMVKITSAEDAEDIKIEYSNVVLADLPPSKE
ILAQVFSGKKPARIYAHFYKEDSDFFSTLPTRDHFKWYFALIAKKGSIDIRRHGNDIAKHKGWSRETVDFMSQVFFELEF
VKINNGVISLNNTSTKRDLADSQTYQLKQAQYTLESDLLYSSFQQLKDWFDEVMQGAVKLEEAKEEKWT

Nucleotide


Download         Length: 2370 bp        

>NTDB_id=1023632 AB2J53_RS20045 WP_368297265.1 3935065..3937434(-) (recJ) [Cytobacillus firmus strain A4]
ATGTTACATTCAAGAACGCGATGGATTGTTCGGCAAACAGAGGAGGATAAAGTACAGCGGCTTTCAAAGGAACTGAATAT
TTCTCCTCTTGCAGCATCGCTTTTAATTAATCGCGGAATGGATACCGTTGATTCAGCACGGTATTTTCTGATGGATAATG
AGCAGGAATTTCATGATCCTTTTTTAATGACAGATATGGCAATTGCTGTTTCCAGAATAAAAGAAGCTATTGAAAAACAG
GAACCTATTTTAATTTTTGGTGATTATGATGCGGATGGTGTGACCAGCACTTCTGTCATGGTGATGGCACTTAAAGATCT
CGGTGCTGCTGTCCAATTCTACATACCGAATCGTTTTACGGAGGGCTATGGGCCAAATATCCCGGCTTTTGAACATGCAG
CCGAAATTGGCATCCGTTTAATTATTACTGTAGATACTGGAATCTCTGCTGTAAATGAAGCAAAAGCTGCAAAAGAGCTT
GGAATGGATTTGATCATTACAGATCACCATGAGCCCGGCCCCGAGCTTCCTGAAGCATTGGCCATTGTGCATCCTAAGCA
TCCGGAGAGCAGATACCCGTTCCGGGAACTTGCCGGTGTTGGGGTAGCATTTAAGCTTGCACATGCGCTTTATGGTGAAA
TGCCTGAACACCTTTTGGAGATAGCTGCGATCGGCACAATTGCTGATCTTGTTTCTTTAACAGGAGAAAACAGACTTATT
GCCAAGCGCGGGATTCAAAAGCTGAAATCAACTAAAAATACAGGTTTGAATGCTTTGTTTAAAGCGGCACGAATCGAGCT
TCCATCCATTAGTGAGGAAACGATCGGCTTTACAATCGGCCCTCGCCTCAATGCAGCAGGAAGGCTTGAAAGCGCGGATC
CTGCTGTGGATCTGCTTTTGACAAATGATCCTTATGAAGCACAAGCCATTGCCGAAGAAATTGAAATGCTGAATAAAGAA
AGGCAATCTATTGTCAATGACATTGCAGCGCAAGCGGTTCATGAGGTTGAAACAAATTTCCCAATCAGTGAGAATTCAGT
TATTGTTGTCGGGAAAGAAGGCTGGAACGCCGGGGTAATCGGAATAGTAGCTTCGAAGCTTGTCGAAAAGTTTTACCGCC
CGGCCATTGTCCTTAGTTATGACAGTGAAAAAGGGCTGGCGAAGGGTTCAGCAAGAAGTATAGAAGGTTTTGATCTGTTC
AAGAATCTTTCTGAATGCCGGGATATCCTGCCTCACTTTGGAGGTCACCCGATGGCAGCGGGTATGACTTTATCCATTGA
TTCGGTTGATGATCTGAGAAGCAGACTGAATACTCTTGCAAATGAACAGCTGAGTGAGGAAGATTTTATTCCTGTAACCT
CTATTGATGGGGTTTTCCATTTAAAAGATATCAATCTTTCTTCCATTGCGGAAATGCAGCTGCTTGCTCCATTCGGGATG
GGAAATGCGAAACCAAAAGTAATGATAAAAGATGCTAATATTGCAGCTATGAGAAAGATCGGATCTGAACAGAACCATAT
TAAAGTCACAATCGAGAATGATGGATCTTCACTTGATGGCATCGGTTTTGGACTTGGGCATTTGCATGAACATATTTCAC
CATACTCCAAGCTGTCCGTTATTGGTGAATTATCCATTAATGAATGGAATAATATTAAAAAACCGCAAATTTTCCTGCAG
GATATGGCAGTCAACTCCTGGCAGCTCTTTGATATCCGGAGTTTAAAACGGCTTGAAAGGCTCCAAAGTGTCATTCCTGA
GGCAAACACAACCTGGTTATTTTTTAATGAGGAATATATTTCTAAATATAAACCATTTGCAGGGGAAAACATGGTGAAAA
TAACATCTGCAGAAGACGCAGAAGACATAAAAATAGAGTACTCAAATGTTGTACTGGCTGATTTGCCGCCTTCAAAGGAA
ATTCTTGCACAGGTTTTCTCCGGAAAAAAACCTGCCAGAATTTATGCTCATTTCTATAAAGAAGACAGTGACTTTTTTAG
CACATTACCAACAAGAGACCATTTTAAATGGTACTTTGCCCTGATTGCTAAAAAAGGTTCAATTGATATCAGGCGTCATG
GGAATGATATAGCGAAGCATAAAGGATGGAGCAGAGAGACAGTAGATTTTATGTCTCAAGTGTTTTTTGAATTGGAATTT
GTTAAAATAAATAATGGTGTCATTTCGTTAAACAATACATCCACTAAACGTGATTTAGCTGATTCACAAACTTATCAGCT
TAAACAGGCACAATATACTCTTGAGAGCGATTTGCTCTATTCATCCTTTCAGCAGCTCAAAGACTGGTTTGATGAAGTTA
TGCAAGGGGCTGTAAAACTTGAGGAGGCAAAGGAAGAAAAATGGACTTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(314-438)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.026

98.352

0.541

Multiple sequence alignment