Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AB2J53_RS19280 Genome accession   NZ_CP162020
Coordinates   3789633..3790703 (-) Length   356 a.a.
NCBI ID   WP_197213501.1    Uniprot ID   -
Organism   Cytobacillus firmus strain A4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3784633..3795703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2J53_RS19235 (AB2J53_19235) - 3785128..3785922 (+) 795 WP_368297189.1 YqhG family protein -
  AB2J53_RS19240 (AB2J53_19240) - 3785966..3786151 (-) 186 WP_035329975.1 YqzE family protein -
  AB2J53_RS19245 (AB2J53_19245) - 3786229..3786726 (-) 498 WP_197213511.1 shikimate kinase -
  AB2J53_RS19250 (AB2J53_19250) comGG 3786739..3787122 (-) 384 WP_197213509.1 competence type IV pilus minor pilin ComGG -
  AB2J53_RS19255 (AB2J53_19255) comGF 3787112..3787528 (-) 417 WP_197213507.1 competence type IV pilus minor pilin ComGF -
  AB2J53_RS19260 (AB2J53_19260) - 3787542..3787871 (-) 330 WP_197213505.1 phosphatase -
  AB2J53_RS19265 (AB2J53_19265) comGD 3787855..3788295 (-) 441 WP_197213968.1 competence type IV pilus minor pilin ComGD -
  AB2J53_RS19270 (AB2J53_19270) comGC 3788292..3788615 (-) 324 WP_197247508.1 competence type IV pilus major pilin ComGC -
  AB2J53_RS19275 (AB2J53_19275) comGB 3788615..3789646 (-) 1032 WP_197213502.1 competence type IV pilus assembly protein ComGB -
  AB2J53_RS19280 (AB2J53_19280) comGA 3789633..3790703 (-) 1071 WP_197213501.1 competence type IV pilus ATPase ComGA Machinery gene
  AB2J53_RS19290 (AB2J53_19290) - 3790972..3791325 (-) 354 WP_048008265.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AB2J53_RS19295 (AB2J53_19295) - 3791572..3792270 (+) 699 WP_197213500.1 metalloregulator ArsR/SmtB family transcription factor -
  AB2J53_RS19300 (AB2J53_19300) - 3792423..3792668 (+) 246 WP_009332938.1 DUF2626 domain-containing protein -
  AB2J53_RS19305 (AB2J53_19305) - 3792703..3793773 (-) 1071 WP_197213967.1 class I SAM-dependent methyltransferase -
  AB2J53_RS19310 (AB2J53_19310) - 3793978..3794613 (-) 636 WP_197213499.1 MBL fold metallo-hydrolase -
  AB2J53_RS19315 (AB2J53_19315) - 3794701..3795180 (+) 480 WP_197213498.1 hypothetical protein -
  AB2J53_RS19320 (AB2J53_19320) - 3795306..3795479 (+) 174 WP_048008269.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40123.30 Da        Isoelectric Point: 8.3383

>NTDB_id=1023628 AB2J53_RS19280 WP_197213501.1 3789633..3790703(-) (comGA) [Cytobacillus firmus strain A4]
MSIIERLAERIIKDAVRSHASDIHIIPRRKDTLIQLRFGSQLTPRLYLPKEECDRLISHFKFTASMDIGEKRRPQSGAYS
LEVDGQMIGLRFSTLPSSHSESLVIRILPQQEQIPFYQISLFPDMTRKMLALLKHAHGLIIFTGPTGSGKTTTLYSLLNE
TSHMFHRNVITLEDPIEKENDLVLQVQVNEKAGVSYSAGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVLSTMH
TRDAEGAVYRLAEFGVNMLEIEQTLVAVTAQRLVELNCPYCEKECSPFCYGYGRWKRASVFELLAGRALNASIKKARGES
SHVKYRTLKEVITKGIALGYIKDTEYSRWVHDDGEA

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1023628 AB2J53_RS19280 WP_197213501.1 3789633..3790703(-) (comGA) [Cytobacillus firmus strain A4]
TTGAGTATAATTGAAAGATTGGCAGAACGAATTATTAAAGACGCGGTCAGAAGCCATGCTTCCGACATCCACATCATTCC
CCGAAGAAAAGACACACTCATTCAGCTGAGGTTCGGCAGCCAATTGACCCCAAGGCTTTATCTTCCCAAAGAAGAGTGTG
ACAGATTGATTTCCCATTTTAAATTTACAGCATCGATGGATATTGGTGAAAAAAGAAGGCCGCAGAGCGGTGCATACTCA
CTTGAAGTAGACGGACAAATGATTGGCCTGCGCTTTTCAACCCTCCCATCAAGCCACAGCGAAAGCCTCGTTATCAGAAT
CCTTCCCCAGCAGGAACAAATTCCTTTTTACCAAATCAGCCTTTTTCCAGACATGACAAGAAAAATGCTGGCACTGCTGA
AGCATGCTCATGGTTTGATTATTTTTACTGGCCCGACCGGCAGCGGTAAAACCACAACTTTGTATTCCCTTCTGAATGAA
ACCTCCCATATGTTCCATCGCAATGTCATCACCCTGGAAGATCCCATTGAAAAAGAGAATGACTTAGTTCTTCAAGTCCA
AGTGAATGAGAAAGCGGGCGTGTCTTATTCTGCAGGCTTAAAAGCAATCCTTCGCCATGACCCCGATATCATTATGGTCG
GTGAAATCCGGGATGCGGAAACGGCGAAGATTGCAGTTAGAGCAGCTCTCACCGGTCACCTTGTTTTAAGCACCATGCAT
ACCCGGGATGCCGAGGGGGCTGTTTACCGTTTAGCTGAGTTTGGAGTGAACATGCTGGAAATTGAGCAGACTTTGGTGGC
GGTCACCGCACAGCGCCTCGTCGAATTGAATTGTCCATATTGTGAAAAGGAATGCTCCCCATTCTGCTATGGATATGGCA
GGTGGAAAAGGGCGAGTGTGTTTGAATTGCTCGCAGGAAGGGCGCTCAATGCCTCCATAAAGAAGGCAAGAGGGGAAAGC
AGCCATGTTAAGTATCGAACACTGAAGGAAGTCATAACTAAAGGGATTGCTCTAGGCTATATAAAGGATACAGAGTACAG
CAGGTGGGTGCATGACGATGGAGAAGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.618

100

0.556
  pilB Glaesserella parasuis strain SC1401

39.714

98.315

0.39

Multiple sequence alignment