Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AB2J53_RS00575 Genome accession   NZ_CP162020
Coordinates   112323..113702 (+) Length   459 a.a.
NCBI ID   WP_035332338.1    Uniprot ID   W7L2B7
Organism   Cytobacillus firmus strain A4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 107323..118702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2J53_RS00555 (AB2J53_00555) - 107438..107899 (+) 462 WP_009336509.1 CtsR family transcriptional regulator -
  AB2J53_RS00560 (AB2J53_00560) - 107923..108471 (+) 549 WP_048011542.1 UvrB/UvrC motif-containing protein -
  AB2J53_RS00565 (AB2J53_00565) - 108473..109552 (+) 1080 WP_048011541.1 protein arginine kinase -
  AB2J53_RS00570 (AB2J53_00570) clpC 109557..112007 (+) 2451 WP_048011540.1 ATP-dependent protease ATP-binding subunit ClpC -
  AB2J53_RS00575 (AB2J53_00575) radA 112323..113702 (+) 1380 WP_035332338.1 DNA repair protein RadA Machinery gene
  AB2J53_RS00580 (AB2J53_00580) disA 113705..114778 (+) 1074 WP_197212317.1 DNA integrity scanning diadenylate cyclase DisA -
  AB2J53_RS00585 (AB2J53_00585) - 114997..116085 (+) 1089 WP_048011538.1 PIN/TRAM domain-containing protein -
  AB2J53_RS00590 (AB2J53_00590) ispD 116171..116857 (+) 687 WP_197212318.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  AB2J53_RS00595 (AB2J53_00595) ispF 116872..117357 (+) 486 WP_048011536.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49533.99 Da        Isoelectric Point: 7.4342

>NTDB_id=1023598 AB2J53_RS00575 WP_035332338.1 112323..113702(+) (radA) [Cytobacillus firmus strain A4]
MAKRKTKFMCQECGYESAKWMGKCPGCGQWNTMVEEVEKPASTRRGAFAHSQGTAIAAKATPITSIETVSEPRIHTDLKE
LNRVLGGGIVRGSLVLIGGDPGIGKSTLLLQVSSQLANRKHSVLYISGEESMRQTKLRADRLGVSSDSLLVYSETSLEEI
SRTIETVNPDFVIIDSIQTIFHPEVTSAPGSVSQVRECTAELMRIGKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKELGLEEVANPSEIFLEERSQGAAGSTVVASMEGTRPVLVEIQALISPTS
FGNPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDKPTKATDCIIGEVGL
TGEVRRVSRIEQRVQEAAKLGFERVILPANNLGGWSAPGGIELIGVSSVGEALKAALGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=1023598 AB2J53_RS00575 WP_035332338.1 112323..113702(+) (radA) [Cytobacillus firmus strain A4]
ATGGCTAAAAGAAAAACGAAATTCATGTGCCAGGAATGCGGCTATGAATCAGCGAAATGGATGGGAAAATGTCCGGGGTG
CGGACAATGGAATACGATGGTGGAAGAGGTAGAGAAGCCAGCTTCAACGAGAAGAGGCGCTTTTGCACATTCACAGGGAA
CGGCCATAGCGGCAAAAGCTACGCCGATTACATCCATTGAAACAGTCAGCGAACCCCGCATACATACCGATTTAAAAGAA
TTAAACCGTGTCCTCGGGGGCGGGATTGTAAGAGGTTCCCTTGTTCTTATCGGTGGAGATCCGGGCATCGGGAAATCAAC
GCTTCTTCTTCAAGTGTCCTCACAGCTTGCGAACAGGAAGCATTCAGTCCTCTATATATCAGGGGAAGAATCCATGCGGC
AGACGAAGCTGCGGGCTGACCGTTTGGGTGTTTCTTCAGACAGTCTGCTCGTTTATTCGGAAACCAGCCTTGAAGAAATC
AGCAGAACGATTGAAACAGTCAACCCTGACTTTGTCATTATCGACTCCATACAGACGATTTTTCATCCGGAAGTGACATC
GGCGCCGGGGAGCGTTTCCCAGGTTCGGGAATGTACCGCTGAACTGATGCGCATTGGCAAAACGAAGGGAATTGCCATTT
TTATCGTAGGGCATGTTACAAAGGAGGGCTCCATTGCGGGTCCAAGACTTCTGGAGCACATGGTGGATACGGTTTTATAC
TTTGAAGGTGAACGCCATCATACATACCGGATTTTACGAGCTGTAAAAAACAGATTCGGGTCAACAAACGAGATGGGCAT
TTTTGAGATGAAGGAACTTGGGCTTGAAGAAGTAGCCAATCCATCTGAAATTTTCCTTGAAGAGCGGTCACAAGGGGCTG
CCGGTTCAACAGTTGTTGCTTCCATGGAGGGGACGAGGCCTGTTCTTGTTGAAATTCAGGCGCTTATTTCCCCGACAAGC
TTTGGAAATCCAAGACGAATGGCAACCGGGATCGATCATAACCGGGTCCCGCTCCTGATGGCCGTTTTGGAAAAACGGGT
CGGAATGCTCCTGCAAAATCAGGATGCCTACTTAAAAGTCGCTGGCGGAGTAAAGCTTGATGAACCGGCAATTGATTTGG
CCATAGCGGTCAGCATTGCGTCTAGCTTTCGGGATAAGCCGACAAAAGCAACAGACTGCATTATTGGAGAAGTAGGACTC
ACAGGCGAGGTCAGGAGAGTCTCTAGGATCGAACAAAGAGTACAGGAGGCGGCCAAGCTGGGCTTTGAACGGGTCATCCT
GCCGGCGAACAATCTTGGAGGCTGGAGCGCGCCGGGAGGCATCGAACTGATCGGAGTTAGCTCTGTTGGTGAAGCGCTAA
AGGCTGCGTTAGGGGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(66-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W7L2B7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.609

100

0.778
  radA Streptococcus pneumoniae Rx1

61.488

99.564

0.612
  radA Streptococcus pneumoniae D39

61.488

99.564

0.612
  radA Streptococcus pneumoniae R6

61.488

99.564

0.612
  radA Streptococcus pneumoniae TIGR4

61.488

99.564

0.612
  radA Streptococcus mitis NCTC 12261

61.488

99.564

0.612
  radA Streptococcus mitis SK321

61.488

99.564

0.612

Multiple sequence alignment