Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB3G31_RS20125 Genome accession   NZ_CP162006
Coordinates   4321160..4322737 (-) Length   525 a.a.
NCBI ID   WP_367847819.1    Uniprot ID   -
Organism   Rhodoferax sp. WC2427     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4316160..4327737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3G31_RS20100 (AB3G31_20100) - 4316326..4316811 (+) 486 WP_367847814.1 nuclear transport factor 2 family protein -
  AB3G31_RS20105 (AB3G31_20105) - 4316865..4317686 (-) 822 WP_367847815.1 UPF0149 family protein -
  AB3G31_RS20110 (AB3G31_20110) - 4317748..4319406 (+) 1659 WP_367847816.1 FMN-binding glutamate synthase family protein -
  AB3G31_RS20115 (AB3G31_20115) - 4319387..4319836 (-) 450 WP_367847817.1 putative toxin-antitoxin system toxin component, PIN family -
  AB3G31_RS20120 (AB3G31_20120) - 4319826..4321034 (-) 1209 WP_367847818.1 class I SAM-dependent RNA methyltransferase -
  AB3G31_RS20125 (AB3G31_20125) rpoN/rpoN1 4321160..4322737 (-) 1578 WP_367847819.1 RNA polymerase factor sigma-54 Machinery gene
  AB3G31_RS20130 (AB3G31_20130) lptB 4322745..4323500 (-) 756 WP_367847820.1 LPS export ABC transporter ATP-binding protein -
  AB3G31_RS20135 (AB3G31_20135) lptA 4323506..4324126 (-) 621 WP_367847821.1 lipopolysaccharide transport periplasmic protein LptA -
  AB3G31_RS20140 (AB3G31_20140) - 4324226..4324879 (-) 654 WP_367847822.1 thiol:disulfide interchange protein DsbA/DsbL -
  AB3G31_RS20145 (AB3G31_20145) - 4324962..4325663 (-) 702 WP_367847823.1 SPOR domain-containing protein -
  AB3G31_RS20150 (AB3G31_20150) argS 4325676..4327349 (-) 1674 WP_367847824.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 525 a.a.        Molecular weight: 57734.10 Da        Isoelectric Point: 4.9946

>NTDB_id=1023458 AB3G31_RS20125 WP_367847819.1 4321160..4322737(-) (rpoN/rpoN1) [Rhodoferax sp. WC2427]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVDQMLDDNPFLERNLDDAPREEFGLAQADTPVSAGDRETESAPT
SVAEYTSETSTEGSNDSESPLTGGDDTSWDGDGTSEMKPDDSEWGGDAPARQNNLGEDGEADATELARNQESLTSFLHRQ
ALALRLGEVDQAALRFLIESLNDDGYLEDDLASLAQGLAGDDLEAQEELVHRFTVALGLLQSLEPAGVGATHLGECLVLQ
LKALQSTMGNDPVRKIALRICRQPMDLLARRDVKRLAQLCHATDADIKLAIALIARLEPKPGRRFVDVERNIVVPDVIVA
KVGRGANAKFRVVLNPEVMPRLRVHDIYANALRQHKGGGKDSSNNAALQQRLQEARWFIKNIQQRFDTILRVSNAIVERQ
KSFFSHGALAMRPLVLREIADELGLHESTISRVTTAKYMATPYGTFELKYFFGSSLGTETGGNASSTAVRALIKQFVASE
DLKKPLSDSQLSDMLKEQGIECARRTVAKYREALRIAPTNLRKAL

Nucleotide


Download         Length: 1578 bp        

>NTDB_id=1023458 AB3G31_RS20125 WP_367847819.1 4321160..4322737(-) (rpoN/rpoN1) [Rhodoferax sp. WC2427]
ATGAAGCAAGGCCTTTCCCTACGCGTTTCCCAGCACCTGGCGCTCACGCCCCAGTTGCAGCAGTCCATCCGCCTGTTGCA
GCTCTCCACCCTGGAGCTGAGCCAGGAAGTGGACCAGATGCTGGACGACAACCCGTTTCTGGAACGCAACCTGGACGACG
CACCCCGCGAAGAGTTTGGCCTGGCCCAGGCCGACACGCCCGTGAGCGCAGGCGACCGCGAAACCGAATCTGCTCCTACT
TCTGTAGCTGAATACACAAGCGAGACCAGCACCGAGGGCTCAAATGACTCAGAGTCGCCGCTGACCGGTGGGGACGACAC
CAGCTGGGATGGCGACGGCACCTCCGAGATGAAGCCCGACGACAGCGAGTGGGGCGGCGACGCACCCGCCCGCCAGAACA
ACCTGGGCGAAGACGGCGAGGCCGATGCCACCGAGCTGGCCCGCAACCAGGAATCGCTGACCAGCTTTTTGCACCGCCAG
GCACTGGCGCTGCGCCTGGGTGAGGTAGACCAGGCGGCGCTGCGCTTCCTGATCGAGTCCCTGAACGACGACGGCTACCT
CGAAGACGACCTGGCCAGCCTGGCCCAGGGCCTGGCGGGCGACGACCTGGAGGCCCAGGAAGAGCTGGTGCACCGCTTCA
CCGTCGCCTTGGGCCTGCTGCAAAGCCTGGAGCCCGCTGGCGTGGGTGCGACCCATCTGGGCGAATGCCTGGTGTTGCAG
CTCAAGGCGTTGCAATCCACCATGGGCAACGACCCGGTGCGCAAGATCGCACTGCGCATCTGCCGCCAGCCCATGGACCT
GCTGGCCCGGCGCGACGTGAAGCGCCTGGCCCAGCTCTGCCACGCCACCGATGCCGACATCAAACTGGCCATTGCGCTGA
TCGCCCGGCTGGAGCCCAAGCCTGGCCGCCGCTTTGTGGATGTGGAGCGCAACATCGTGGTGCCCGACGTGATCGTGGCC
AAAGTGGGGCGGGGTGCCAACGCCAAGTTCCGGGTGGTGCTGAACCCCGAGGTCATGCCGCGCCTGCGCGTGCACGACAT
CTACGCCAACGCCCTGCGCCAGCACAAGGGTGGCGGCAAGGACTCTTCGAACAACGCCGCCCTGCAGCAGCGCTTGCAGG
AAGCCCGTTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCTTGCGGGTCAGTAACGCCATCGTGGAGCGGCAA
AAAAGCTTCTTCAGCCACGGCGCGCTGGCCATGCGCCCCTTGGTGCTGCGCGAGATTGCCGACGAACTGGGCCTGCACGA
GTCCACCATCAGCCGCGTCACCACGGCCAAGTACATGGCTACGCCCTACGGCACCTTCGAGCTGAAATACTTCTTCGGCT
CATCCCTGGGCACCGAGACGGGCGGCAATGCCTCCAGCACGGCGGTGCGCGCGCTCATCAAGCAGTTCGTTGCCTCCGAA
GACCTGAAAAAGCCGCTCAGCGACAGCCAACTGTCGGATATGCTGAAAGAGCAGGGCATCGAATGCGCCCGCCGCACGGT
AGCCAAGTACCGCGAAGCCTTGCGGATCGCGCCGACGAATCTGCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(367-523)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(144-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.019

100

0.535

Multiple sequence alignment