Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AB3G53_RS02460 Genome accession   NZ_CP161999
Coordinates   527261..528061 (+) Length   266 a.a.
NCBI ID   WP_001984465.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain SIMBA061     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 522261..533061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3G53_RS02435 (AB3G53_02435) - 523171..524469 (+) 1299 WP_001991191.1 glycosyltransferase family 1 protein -
  AB3G53_RS02440 (AB3G53_02440) - 524494..525045 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  AB3G53_RS02445 (AB3G53_02445) iscX 525145..525345 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  AB3G53_RS02450 (AB3G53_02450) ndk 525459..525890 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  AB3G53_RS02455 (AB3G53_02455) rlmN 526013..527245 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AB3G53_RS02460 (AB3G53_02460) pilF 527261..528061 (+) 801 WP_001984465.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AB3G53_RS02465 (AB3G53_02465) - 528052..528843 (+) 792 WP_057094185.1 helix-turn-helix domain-containing protein -
  AB3G53_RS02470 (AB3G53_02470) ispG 528865..529980 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AB3G53_RS02475 (AB3G53_02475) hisS 529977..531269 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  AB3G53_RS02480 (AB3G53_02480) - 531298..531996 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30084.40 Da        Isoelectric Point: 9.2307

>NTDB_id=1023377 AB3G53_RS02460 WP_001984465.1 527261..528061(+) (pilF) [Acinetobacter baumannii strain SIMBA061]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1023377 AB3G53_RS02460 WP_001984465.1 527261..528061(+) (pilF) [Acinetobacter baumannii strain SIMBA061]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722

Multiple sequence alignment