Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | AB2M35_RS13750 | Genome accession | NZ_CP161902 |
| Coordinates | 2566988..2567398 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis strain DYJ22 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2562355..2590260 | 2566988..2567398 | within | 0 |
Gene organization within MGE regions
Location: 2562355..2590260
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AB2M35_RS13725 (AB2M35_13725) | yqeF | 2562522..2563253 (-) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| AB2M35_RS13730 (AB2M35_13730) | cwlH | 2563505..2564257 (-) | 753 | WP_069837642.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| AB2M35_RS13735 (AB2M35_13735) | yqeD | 2564444..2565070 (+) | 627 | WP_014480319.1 | TVP38/TMEM64 family protein | - |
| AB2M35_RS13740 (AB2M35_13740) | gnd | 2565089..2565982 (-) | 894 | WP_069837643.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| AB2M35_RS13745 (AB2M35_13745) | yqeB | 2566233..2566955 (+) | 723 | WP_014480321.1 | hypothetical protein | - |
| AB2M35_RS13750 (AB2M35_13750) | nucA/comI | 2566988..2567398 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| AB2M35_RS13755 (AB2M35_13755) | sigK | 2567594..2568322 (+) | 729 | WP_013308023.1 | RNA polymerase sporulation sigma factor SigK | - |
| AB2M35_RS13760 (AB2M35_13760) | - | 2568322..2568420 (+) | 99 | WP_031600702.1 | hypothetical protein | - |
| AB2M35_RS13765 (AB2M35_13765) | - | 2568417..2568629 (-) | 213 | Protein_2664 | recombinase family protein | - |
| AB2M35_RS13770 (AB2M35_13770) | fumC | 2568848..2570236 (-) | 1389 | WP_014480325.1 | class II fumarate hydratase | - |
| AB2M35_RS13775 (AB2M35_13775) | - | 2570403..2571293 (+) | 891 | WP_014480326.1 | LysR family transcriptional regulator | - |
| AB2M35_RS13780 (AB2M35_13780) | - | 2572235..2572630 (+) | 396 | WP_014480327.1 | VOC family protein | - |
| AB2M35_RS13785 (AB2M35_13785) | - | 2573370..2574497 (-) | 1128 | WP_014480328.1 | Rap family tetratricopeptide repeat protein | - |
| AB2M35_RS13790 (AB2M35_13790) | - | 2574679..2576605 (+) | 1927 | Protein_2669 | ribonuclease YeeF family protein | - |
| AB2M35_RS13795 (AB2M35_13795) | - | 2576619..2576906 (+) | 288 | WP_014480331.1 | hypothetical protein | - |
| AB2M35_RS13800 (AB2M35_13800) | - | 2577309..2577749 (+) | 441 | WP_014480332.1 | SMI1/KNR4 family protein | - |
| AB2M35_RS13805 (AB2M35_13805) | - | 2577848..2578300 (+) | 453 | WP_014480333.1 | SMI1/KNR4 family protein | - |
| AB2M35_RS13810 (AB2M35_13810) | cdiI | 2578397..2578756 (+) | 360 | WP_014480334.1 | ribonuclease toxin immunity protein CdiI | - |
| AB2M35_RS13815 (AB2M35_13815) | - | 2578861..2579340 (+) | 480 | WP_224588637.1 | hypothetical protein | - |
| AB2M35_RS13820 (AB2M35_13820) | - | 2579644..2579847 (-) | 204 | WP_123772462.1 | hypothetical protein | - |
| AB2M35_RS13825 (AB2M35_13825) | - | 2579927..2580160 (+) | 234 | WP_224588641.1 | hypothetical protein | - |
| AB2M35_RS13830 (AB2M35_13830) | atxG | 2580417..2580994 (+) | 578 | Protein_2677 | suppressor of fused domain protein | - |
| AB2M35_RS13835 (AB2M35_13835) | - | 2581104..2581394 (+) | 291 | WP_014480337.1 | contact-dependent growth inhibition system immunity protein | - |
| AB2M35_RS13840 (AB2M35_13840) | istA | 2582235..2583782 (+) | 1548 | WP_014480339.1 | IS21 family transposase | - |
| AB2M35_RS13845 (AB2M35_13845) | istB | 2583779..2584537 (+) | 759 | WP_014479891.1 | IS21-like element helper ATPase IstB | - |
| AB2M35_RS13850 (AB2M35_13850) | - | 2584855..2584952 (-) | 98 | Protein_2681 | N-acetylmuramoyl-L-alanine amidase | - |
| AB2M35_RS13855 (AB2M35_13855) | - | 2585157..2585216 (+) | 60 | WP_076458313.1 | putative holin-like toxin | - |
| AB2M35_RS13860 (AB2M35_13860) | - | 2585503..2585939 (-) | 437 | Protein_2683 | phage tail tube protein | - |
| AB2M35_RS13865 (AB2M35_13865) | terS | 2585937..2586502 (-) | 566 | Protein_2684 | phage terminase small subunit | - |
| AB2M35_RS13870 (AB2M35_13870) | - | 2586629..2586934 (+) | 306 | WP_123772463.1 | hypothetical protein | - |
| AB2M35_RS13875 (AB2M35_13875) | - | 2587107..2587172 (-) | 66 | Protein_2686 | hypothetical protein | - |
| AB2M35_RS13880 (AB2M35_13880) | - | 2587327..2587806 (-) | 480 | WP_014480344.1 | hypothetical protein | - |
| AB2M35_RS13885 (AB2M35_13885) | - | 2588423..2588689 (+) | 267 | WP_033881358.1 | hypothetical protein | - |
| AB2M35_RS13890 (AB2M35_13890) | - | 2588828..2588980 (-) | 153 | WP_049832653.1 | XtrA/YqaO family protein | - |
| AB2M35_RS13895 (AB2M35_13895) | - | 2589063..2589185 (-) | 123 | Protein_2690 | RusA family crossover junction endodeoxyribonuclease | - |
| AB2M35_RS13900 (AB2M35_13900) | - | 2589148..2589396 (-) | 249 | Protein_2691 | hypothetical protein | - |
| AB2M35_RS13905 (AB2M35_13905) | - | 2589541..2589771 (-) | 231 | WP_224588644.1 | hypothetical protein | - |
| AB2M35_RS13910 (AB2M35_13910) | - | 2590080..2590260 (-) | 181 | Protein_2693 | hypothetical protein | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=1023006 AB2M35_RS13750 WP_009967785.1 2566988..2567398(-) (nucA/comI) [Bacillus subtilis strain DYJ22]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=1023006 AB2M35_RS13750 WP_009967785.1 2566988..2567398(-) (nucA/comI) [Bacillus subtilis strain DYJ22]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |