Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   AB2M35_RS00020 Genome accession   NZ_CP161902
Coordinates   2360..3472 (+) Length   370 a.a.
NCBI ID   WP_014478578.1    Uniprot ID   -
Organism   Bacillus subtilis strain DYJ22     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..8472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2M35_RS00010 (AB2M35_00010) dnaN 862..1998 (+) 1137 WP_003226811.1 DNA polymerase III subunit beta -
  AB2M35_RS00015 (AB2M35_00015) rlbA 2129..2344 (+) 216 WP_003226810.1 ribosome maturation protein RlbA -
  AB2M35_RS00020 (AB2M35_00020) recF 2360..3472 (+) 1113 WP_014478578.1 DNA replication/repair protein RecF Machinery gene
  AB2M35_RS00025 (AB2M35_00025) remB 3490..3735 (+) 246 WP_003219266.1 extracellular matrix regulator RemB -
  AB2M35_RS00030 (AB2M35_00030) gyrB 3790..5706 (+) 1917 WP_003226808.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  AB2M35_RS00035 (AB2M35_00035) gyrA 5917..8382 (+) 2466 WP_014475555.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42304.34 Da        Isoelectric Point: 7.3660

>NTDB_id=1022954 AB2M35_RS00020 WP_014478578.1 2360..3472(+) (recF) [Bacillus subtilis strain DYJ22]
MYIQNLELSSYRNYDHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLIEVAAKVVVKRLQFTAQLEKWAQPIHAGISRGLEELTLKYHTALDVSDPLDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGALVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1022954 AB2M35_RS00020 WP_014478578.1 2360..3472(+) (recF) [Bacillus subtilis strain DYJ22]
TTGTATATCCAGAACTTAGAACTTTCATCTTACCGCAACTACGACCATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATCGGAGAAAACGCCCAGGGGAAGACAAACCTCATGGAGGCGATCTATGTCTTGTCCATGGCGAAATCGCACCGGA
CATCAAATGACAAAGAACTTATACGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCG
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATTGAACAGCAAAAGCTCAGCCAGTA
TGTCGGGGCCCTCAACACCATTATGTTCGCGCCGGAAGATTTAAATCTTGTAAAGGGAAGCCCTCAAGTGAGAAGGCGGT
TTCTTGACATGGAAATTGGACAGGTTTCTCCCGTCTATCTTCATGATCTTTCTCTTTACCAGAAAATCCTTTCCCAGCGG
AATCATTTTTTGAAACAGCTGCAAACAAGAAAACAAACTGACCGGACGATGCTCGATGTTCTGACCGATCAGCTTATAGA
AGTTGCAGCAAAAGTCGTCGTAAAACGCCTGCAGTTTACAGCACAGCTCGAGAAATGGGCGCAGCCCATCCATGCAGGCA
TCTCAAGAGGGCTTGAAGAACTTACCCTGAAATACCATACAGCTCTTGATGTATCAGATCCCCTAGATTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATTGAGCGTGGTGTGACGCTGTCAGGGCCTCATCG
CGATGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGTTCTCAAGGACAGCAGCGAACGACGGCGTTGT
CCCTTAAGCTGGCGGAGATTGACCTGATCCATGAAGAAATCGGAGAATATCCCATTTTACTATTGGATGATGTACTGAGT
GAACTGGATGATTATCGCCAGTCACACTTGCTTCATACGATCCAAGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGTCAAGCAGGAATGTTCCGTGTGCAAAATGGTGCGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

99.459

100

0.995

Multiple sequence alignment