Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   U2F28_RS00200 Genome accession   NZ_CP160635
Coordinates   46466..48094 (-) Length   542 a.a.
NCBI ID   WP_031870018.1    Uniprot ID   -
Organism   Staphylococcus aureus strain BSN44     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 39702..54743 46466..48094 within 0


Gene organization within MGE regions


Location: 39702..54743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2F28_RS00165 (U2F28_000165) mecA 39702..41711 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  U2F28_RS00170 (U2F28_000170) - 41808..42794 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  U2F28_RS00175 (U2F28_000175) - 42766..43026 (+) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  U2F28_RS00180 (U2F28_000180) - 43017..44624 (-) 1608 Protein_33 IS1182 family transposase -
  U2F28_RS00185 (U2F28_000185) - 44676..45182 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  U2F28_RS00190 (U2F28_000190) - 45263..45507 (-) 245 Protein_35 DUF960 family protein -
  U2F28_RS00195 (U2F28_000195) - 45594..45944 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  U2F28_RS00200 (U2F28_000200) ccrB 46466..48094 (-) 1629 WP_031870018.1 cassette chromosome recombinase CcrB Machinery gene
  U2F28_RS00205 (U2F28_000205) ccrA 48116..49465 (-) 1350 WP_031870019.1 cassette chromosome recombinase CcrA Machinery gene
  U2F28_RS00210 (U2F28_000210) - 49699..51492 (-) 1794 WP_000195104.1 cassette chromosome replicative helicase -
  U2F28_RS00215 (U2F28_000215) - 51492..51785 (-) 294 WP_001587828.1 cassette chromosome ssDNA-binding protein -
  U2F28_RS00220 (U2F28_000220) - 52020..53159 (+) 1140 Protein_41 IS3 family transposase -
  U2F28_RS00225 (U2F28_000225) - 53229..54743 (-) 1515 WP_001587831.1 hypothetical protein -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62637.79 Da        Isoelectric Point: 9.8739

>NTDB_id=1021784 U2F28_RS00200 WP_031870018.1 46466..48094(-) (ccrB) [Staphylococcus aureus strain BSN44]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPNSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKTLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=1021784 U2F28_RS00200 WP_031870018.1 46466..48094(-) (ccrB) [Staphylococcus aureus strain BSN44]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGATGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAGGTCAAAGATATATACGCTGATCGTGGTA
TCTCAGGTAAATCTATGAACAGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATCGACTGTGTTATG
GTATACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTGTCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAAATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAATGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCTAATAGTAAACATGAACTGATGATTAATCAACATGAAGCTAATATTGTGAAATA
TATATTCGAGTCCTATGCCAAAGGACATGGCTATCGTAAAATAGCCAATGCATTAAATCACAAAGGCTATGTCACTAAAA
AGGGTAAACCTTTTAGTATTAGTTCAATCACATACATCTTAGCTAACCCTTTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAGAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACTCTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAAATAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGACTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

99.262

100

0.993

Multiple sequence alignment