Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABZP31_RS02105 Genome accession   NZ_CP160437
Coordinates   454195..454998 (+) Length   267 a.a.
NCBI ID   WP_005016505.1    Uniprot ID   A0A2T1J2S1
Organism   Acinetobacter sp. FL     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 449195..459998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZP31_RS02080 (ABZP31_02080) - 450121..451398 (+) 1278 WP_174498840.1 glycosyltransferase family 1 protein -
  ABZP31_RS02085 (ABZP31_02085) - 451427..451978 (+) 552 WP_075040828.1 phosphatase PAP2 family protein -
  ABZP31_RS02090 (ABZP31_02090) iscX 452066..452266 (+) 201 WP_005016518.1 Fe-S cluster assembly protein IscX -
  ABZP31_RS02095 (ABZP31_02095) ndk 452381..452812 (+) 432 WP_005016515.1 nucleoside-diphosphate kinase -
  ABZP31_RS02100 (ABZP31_02100) rlmN 452935..454170 (+) 1236 WP_119878352.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABZP31_RS02105 (ABZP31_02105) pilF 454195..454998 (+) 804 WP_005016505.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABZP31_RS02110 (ABZP31_02110) - 454989..455792 (+) 804 WP_034670256.1 helix-turn-helix domain-containing protein -
  ABZP31_RS02115 (ABZP31_02115) ispG 455815..456930 (+) 1116 WP_005016500.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABZP31_RS02120 (ABZP31_02120) hisS 456927..458222 (+) 1296 WP_005016497.1 histidine--tRNA ligase -
  ABZP31_RS02125 (ABZP31_02125) - 458254..458958 (+) 705 WP_248133917.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30279.57 Da        Isoelectric Point: 9.5400

>NTDB_id=1021121 ABZP31_RS02105 WP_005016505.1 454195..454998(+) (pilF) [Acinetobacter sp. FL]
MRKPVYKTFVLLTVTLSALLVTACQTPDTLSKDPEKAVKVRTQLAAEYIKSGDLDAAKRALDQALEVDSRDATANMMMGV
LLQQEGSQLNLDKAESYFRRSISIEPKNAQARNNYGAYLYQIGRYKDAIEQLEIAGATLGYEQRYQALENLGRIYLKLGN
VANAEKTFKQALQANRDSSISMLELAEIFYLRQQIPAATQLYEQYVRRVGQKNQGARALWIGIRIARANDDKMGTQVLVN
QLRALFPDSQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=1021121 ABZP31_RS02105 WP_005016505.1 454195..454998(+) (pilF) [Acinetobacter sp. FL]
TTGAGAAAGCCGGTGTATAAAACATTTGTATTACTTACAGTGACTTTGTCGGCCTTGCTTGTTACTGCTTGCCAGACTCC
TGATACCCTCAGTAAGGATCCAGAAAAGGCAGTCAAGGTTCGAACCCAGCTGGCAGCGGAATATATTAAATCGGGTGATC
TGGATGCTGCTAAACGGGCACTAGACCAGGCACTTGAAGTTGATTCCAGAGACGCCACTGCGAACATGATGATGGGAGTA
CTGCTCCAGCAAGAGGGGAGTCAGCTAAATCTGGACAAGGCTGAGAGCTATTTCAGACGCTCTATTTCAATTGAGCCTAA
AAATGCACAGGCGCGTAATAATTATGGCGCCTATTTGTACCAGATCGGTCGTTATAAAGATGCGATCGAACAACTGGAAA
TTGCTGGTGCAACTTTAGGCTATGAGCAGCGTTACCAGGCGCTTGAAAATCTGGGACGAATTTATCTTAAGCTCGGCAAT
GTTGCGAATGCTGAAAAAACATTCAAACAGGCATTGCAAGCTAACCGCGATTCTTCCATTTCAATGTTAGAGTTAGCGGA
AATTTTCTATTTGCGGCAACAGATTCCTGCTGCTACACAGCTTTATGAACAATATGTGCGTCGTGTAGGGCAAAAGAATC
AGGGTGCGCGTGCTCTTTGGATCGGAATTCGAATTGCGCGTGCCAATGATGATAAAATGGGAACACAAGTTTTGGTGAAC
CAGTTACGTGCCTTGTTCCCGGACAGCCAAGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(44-82)

Tetratricopeptide repeat (TPR_16)

(150-208)

Tetratricopeptide repeat (TPR_17)

(98-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T1J2S1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.401

100

0.794
  pilF Acinetobacter baylyi ADP1

73.81

94.382

0.697

Multiple sequence alignment