Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB0A83_RS04090 Genome accession   NZ_CP160405
Coordinates   863204..864640 (+) Length   478 a.a.
NCBI ID   WP_150046974.1    Uniprot ID   -
Organism   Methylomonas sp. HYX-M1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 858204..869640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0A83_RS04060 (AB0A83_04060) - 858916..859734 (-) 819 WP_150046962.1 ABC transporter ATP-binding protein -
  AB0A83_RS04065 (AB0A83_04065) - 859874..860854 (+) 981 WP_150046964.1 SIS domain-containing protein -
  AB0A83_RS04070 (AB0A83_04070) kdsC 860861..861406 (+) 546 WP_367155473.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AB0A83_RS04075 (AB0A83_04075) lptC 861403..861969 (+) 567 WP_150046967.1 LPS export ABC transporter periplasmic protein LptC -
  AB0A83_RS04080 (AB0A83_04080) lptA 861956..862462 (+) 507 WP_150046970.1 lipopolysaccharide transport periplasmic protein LptA -
  AB0A83_RS04085 (AB0A83_04085) lptB 862464..863189 (+) 726 WP_150046972.1 LPS export ABC transporter ATP-binding protein -
  AB0A83_RS04090 (AB0A83_04090) rpoN/rpoN1 863204..864640 (+) 1437 WP_150046974.1 RNA polymerase factor sigma-54 Machinery gene
  AB0A83_RS04095 (AB0A83_04095) raiA 864682..864972 (+) 291 WP_150046976.1 ribosome-associated translation inhibitor RaiA -
  AB0A83_RS04100 (AB0A83_04100) rapZ 865030..865887 (+) 858 WP_150046978.1 RNase adapter RapZ -
  AB0A83_RS04105 (AB0A83_04105) mgtE 865919..867277 (+) 1359 WP_367155474.1 magnesium transporter -
  AB0A83_RS04110 (AB0A83_04110) minC 867350..868090 (+) 741 WP_150046982.1 septum site-determining protein MinC -
  AB0A83_RS04115 (AB0A83_04115) minD 868112..868924 (+) 813 WP_150046983.1 septum site-determining protein MinD -
  AB0A83_RS04120 (AB0A83_04120) minE 868924..869178 (+) 255 WP_150051785.1 cell division topological specificity factor MinE -
  AB0A83_RS04125 (AB0A83_04125) - 869256..869453 (+) 198 WP_150046985.1 winged helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 54023.09 Da        Isoelectric Point: 4.4990

>NTDB_id=1020998 AB0A83_RS04090 WP_150046974.1 863204..864640(+) (rpoN/rpoN1) [Methylomonas sp. HYX-M1]
MKQSLQLRIGQSLTMTPQLQQAIKLLQMSTLDLQQEIQQALESNMMLEIEEDDLPTLEYRDKKADNNDQMTSEGSQTDMP
DELPVDVSWDDVYESTLPTGEDGSDSSEFETFRGKTQTLQDHLLWQLDLTHLSERDYAIATAIIDAINDDGYITGELHDI
YQGLLEQLDDLEMDEVQAVLHMIQNFDPVGVGALNLADCLCLQLRQLPDDTPYKEDALEFAGRHLELLTSCDQARLIKRV
GFSEEQLKGVLALIRSLDPKPGARLQEADVEYVVPDVFVAKVKDQWQVALNPDIAPKLRINPFYSGMIKRADNSQDNVSM
KNHLQEARWFIKSLHSRNDTLLRVAKSIVEKQGEFFEHGPIAMKAMVLRDIAEELDLHESTISRVTTQKYMHTPQGVIEF
KYFFSSHVSTDSGGECSATAIRALIKELVGNENPAKPLSDSKISDLLNEKGINVARRTIAKYREAMSIPSTSQRKRLI

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=1020998 AB0A83_RS04090 WP_150046974.1 863204..864640(+) (rpoN/rpoN1) [Methylomonas sp. HYX-M1]
ATGAAGCAATCCTTACAACTTCGCATCGGTCAAAGCTTGACCATGACGCCGCAGCTGCAGCAGGCCATCAAGCTGCTGCA
GATGTCGACCTTGGACCTGCAACAAGAAATTCAGCAGGCACTCGAATCCAACATGATGCTGGAAATCGAAGAAGACGATC
TGCCGACGTTGGAATATCGGGATAAGAAAGCCGACAACAACGACCAAATGACCAGCGAGGGTTCGCAAACCGATATGCCG
GACGAACTGCCGGTCGACGTTAGTTGGGACGACGTTTACGAAAGCACGCTGCCGACCGGAGAAGATGGCTCGGACAGTAG
CGAATTCGAAACTTTTCGCGGTAAAACCCAAACCTTGCAAGATCATTTGCTTTGGCAATTGGATTTAACGCATCTCTCCG
AACGCGACTACGCAATAGCCACCGCCATTATCGACGCCATCAACGATGACGGCTACATCACCGGCGAATTGCACGATATT
TACCAAGGTCTGCTGGAACAATTGGACGATTTGGAAATGGACGAAGTGCAGGCCGTGCTGCACATGATTCAAAATTTCGA
TCCGGTCGGCGTGGGCGCGTTGAACTTGGCCGATTGCCTGTGTTTACAGCTAAGGCAACTGCCGGACGATACCCCTTACA
AGGAAGATGCTCTGGAATTTGCCGGCCGCCATCTGGAGTTGCTGACCAGTTGCGATCAAGCCCGTTTGATCAAACGGGTT
GGTTTCAGCGAAGAACAGCTGAAAGGTGTTCTGGCCCTGATTCGCAGCCTGGACCCTAAGCCGGGCGCGAGGCTGCAGGA
AGCCGACGTCGAATACGTGGTCCCCGACGTGTTCGTTGCCAAGGTAAAAGACCAATGGCAAGTGGCCTTGAATCCCGACA
TCGCGCCCAAATTGCGCATCAATCCGTTTTATTCGGGCATGATCAAACGGGCCGACAACAGTCAAGACAATGTCAGCATG
AAGAACCATTTGCAGGAAGCACGCTGGTTCATCAAAAGTTTGCACAGCCGCAACGATACCTTGTTGCGGGTGGCCAAAAG
CATTGTGGAAAAACAAGGCGAGTTCTTCGAGCATGGCCCCATCGCGATGAAAGCCATGGTGCTAAGAGACATCGCCGAAG
AACTGGATTTGCATGAATCGACCATCTCGCGGGTCACCACGCAAAAATACATGCACACTCCGCAAGGCGTCATCGAATTC
AAATATTTCTTTTCCAGCCACGTCAGCACCGACAGCGGCGGCGAATGCTCGGCTACGGCAATACGGGCGTTAATCAAAGA
ACTGGTCGGCAACGAAAACCCGGCCAAACCCTTAAGCGATAGTAAAATATCCGACTTATTGAACGAGAAAGGCATCAATG
TGGCGCGTCGTACCATCGCCAAATACCGCGAAGCCATGTCGATTCCTTCCACCAGCCAAAGGAAACGGCTAATTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-476)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(109-304)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.23

100

0.487

Multiple sequence alignment