Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AAFM42_RS05870 Genome accession   NZ_CP160329
Coordinates   1142263..1143630 (-) Length   455 a.a.
NCBI ID   WP_053258110.1    Uniprot ID   A0A1E4XKW2
Organism   Pseudomonas fluorescens strain PFX1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1137263..1148630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFM42_RS05845 (AAFM42_05805) yjiA 1137707..1138666 (-) 960 WP_053258107.1 GTPase -
  AAFM42_RS05850 (AAFM42_05810) - 1138746..1138943 (-) 198 WP_003194308.1 YbdD/YjiX family protein -
  AAFM42_RS05855 (AAFM42_05815) - 1138995..1141061 (-) 2067 WP_394546598.1 carbon starvation CstA family protein -
  AAFM42_RS05860 (AAFM42_05820) - 1141116..1141484 (+) 369 WP_053258109.1 PilZ domain-containing protein -
  AAFM42_RS05865 (AAFM42_05825) - 1141497..1142231 (-) 735 WP_053258281.1 hypothetical protein -
  AAFM42_RS05870 (AAFM42_05830) radA 1142263..1143630 (-) 1368 WP_053258110.1 DNA repair protein RadA Machinery gene
  AAFM42_RS05875 (AAFM42_05835) - 1143667..1144206 (-) 540 WP_053258111.1 ankyrin repeat domain-containing protein -
  AAFM42_RS05880 (AAFM42_05840) katB 1144249..1145790 (-) 1542 WP_053258112.1 catalase KatB -
  AAFM42_RS05885 (AAFM42_05845) mscL 1145830..1146246 (+) 417 WP_053258113.1 large-conductance mechanosensitive channel protein MscL -
  AAFM42_RS05890 (AAFM42_05850) - 1146272..1147048 (-) 777 WP_053258114.1 ferredoxin--NADP reductase -
  AAFM42_RS05895 (AAFM42_05855) - 1147112..1147822 (+) 711 WP_053258115.1 LuxR family transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48759.23 Da        Isoelectric Point: 6.8987

>NTDB_id=1020506 AAFM42_RS05870 WP_053258110.1 1142263..1143630(-) (radA) [Pseudomonas fluorescens strain PFX1]
MAKAKRMYGCTECGATFPKWAGQCSECGAWNTLTETMIESGGAAAPTGRAGWTGQQAQIKTLAEVSVEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCSIASRMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIESIIA
TARIEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFAMTDRGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKESPPGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1020506 AAFM42_RS05870 WP_053258110.1 1142263..1143630(-) (radA) [Pseudomonas fluorescens strain PFX1]
ATGGCAAAGGCCAAGCGCATGTACGGCTGCACGGAGTGCGGCGCGACCTTTCCCAAGTGGGCCGGCCAATGCTCCGAGTG
TGGCGCGTGGAACACCCTGACTGAAACCATGATCGAAAGCGGCGGCGCCGCAGCCCCCACTGGCCGAGCCGGCTGGACCG
GGCAGCAGGCGCAGATCAAGACCTTGGCCGAAGTCAGCGTCGAAGAGATCCCGCGTTTCTCCACGGCGTCCGGTGAGCTG
GACCGCGTGCTCGGCGGTGGCCTGGTGGACGGTTCGGTGGTGCTGATCGGGGGCGACCCCGGCATCGGTAAATCGACCAT
CCTGTTGCAAACCCTGTGCAGCATCGCCAGTCGCATGCCGGCGCTGTATGTCACCGGCGAAGAATCCCAACAGCAAGTGG
CCATGCGCGCGCGTCGCCTGGGCCTGCCCCAGGATCAACTGCGGGTGATGACCGAAACCTGCATCGAAAGCATCATCGCC
ACGGCGCGTATCGAAAAACCCAAGGTCATGGTGATCGACTCGATCCAGACGATTTTCACCGAGCAGTTGCAGTCGGCACC
GGGCGGCGTATCCCAGGTGCGTGAGAGCGCGGCGCTGCTGGTGCGGTATGCCAAGCAAAGCGGCACGGCGATCTTCCTGG
TCGGCCACGTGACCAAAGAAGGCGCGCTGGCCGGGCCACGGGTGCTTGAGCATATGGTCGACACGGTGCTGTATTTCGAA
GGCGAATCCGATGGCCGTTTGCGTTTGCTGCGGGCCGTGAAGAACCGCTTTGGCGCGGTCAATGAGTTGGGCGTGTTTGC
CATGACCGACCGGGGGTTGAAAGAAGTCTCCAACCCGTCAGCCATTTTTCTCACCCGCGCCCAGGAAGAAGTGCCGGGAA
GTGTGGTGATGGCGACGTGGGAAGGCACTCGCCCGATGTTGGTGGAAGTGCAGGCGCTGGTGGATGACAGCCACTTGGCC
AACCCGCGCCGGGTCACCCTCGGCCTGGATCAGAACCGCCTGGCGATGTTGCTCGCTGTGCTCCACCGTCACGGCGGTAT
TCCCACCCATGACCAGGACGTCTTCCTCAACGTGGTCGGCGGGGTCAAGGTGCTGGAAACCGCCTCCGACCTGGCGTTGA
TGGCGGCGGTGATGTCCAGCCTGCGCAATCGCCCGCTGCCCCATGACTTGCTGGTATTCGGCGAAGTCGGCCTGTCGGGC
GAGGTACGCCCGGTGCCGAGTGGCCAGGAGCGCCTGAAAGAAGCGGCCAAGCACGGTTTCAAACGCGCGATCGTGCCCAA
GGGCAATGCGCCGAAGGAATCGCCGCCGGGCTTGCAGGTCATTGCAGTCACGCGCCTGGAGCAGGCGCTGGATGCGCTGT
TCGAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(80-221)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E4XKW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.453

100

0.497
  radA Streptococcus mitis SK321

46.93

100

0.47
  radA Streptococcus mitis NCTC 12261

46.711

100

0.468
  radA Streptococcus pneumoniae Rx1

46.711

100

0.468
  radA Streptococcus pneumoniae D39

46.711

100

0.468
  radA Streptococcus pneumoniae R6

46.711

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.711

100

0.468

Multiple sequence alignment