Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AB0R67_RS20005 Genome accession   NZ_CP160237
Coordinates   3954492..3954926 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain JJ1216     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3949492..3959926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R67_RS19990 (AB0R67_19995) - 3950670..3951227 (+) 558 WP_000062063.1 PadR family transcriptional regulator -
  AB0R67_RS19995 (AB0R67_20000) metE 3951739..3954027 (+) 2289 WP_001007615.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  AB0R67_RS20000 (AB0R67_20005) - 3954066..3954455 (-) 390 WP_000424023.1 competence protein ComJ -
  AB0R67_RS20005 (AB0R67_20010) nucA/comI 3954492..3954926 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  AB0R67_RS20010 (AB0R67_20015) - 3955153..3955902 (+) 750 WP_000388467.1 DUF3967 domain-containing protein -
  AB0R67_RS20015 (AB0R67_20020) kinB 3955941..3957215 (-) 1275 WP_000420372.1 sporulation sensor histidine kinase KinB -
  AB0R67_RS20020 (AB0R67_20025) - 3957526..3957726 (+) 201 WP_000929276.1 hypothetical protein -
  AB0R67_RS20025 (AB0R67_20030) - 3957847..3959010 (+) 1164 WP_023522939.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=1020386 AB0R67_RS20005 WP_000811494.1 3954492..3954926(-) (nucA/comI) [Bacillus thuringiensis strain JJ1216]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1020386 AB0R67_RS20005 WP_000811494.1 3954492..3954926(-) (nucA/comI) [Bacillus thuringiensis strain JJ1216]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCGTATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGGGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAGTTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment