Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   AB0R75_RS11535 Genome accession   NZ_CP160232
Coordinates   2281155..2281925 (-) Length   256 a.a.
NCBI ID   WP_012010612.1    Uniprot ID   A8FFB2
Organism   Bacillus pumilus strain JJ1622     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2276155..2286925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R75_RS11515 (AB0R75_11515) - 2276248..2277069 (-) 822 WP_108611178.1 pyruvate, water dikinase regulatory protein -
  AB0R75_RS11520 (AB0R75_11520) - 2277095..2277733 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  AB0R75_RS11525 (AB0R75_11525) glyS 2277900..2279945 (-) 2046 WP_335349165.1 glycine--tRNA ligase subunit beta -
  AB0R75_RS11530 (AB0R75_11530) glyQ 2279938..2280825 (-) 888 WP_003217487.1 glycine--tRNA ligase subunit alpha -
  AB0R75_RS11535 (AB0R75_11535) recO 2281155..2281925 (-) 771 WP_012010612.1 DNA repair protein RecO Machinery gene
  AB0R75_RS11540 (AB0R75_11540) - 2281962..2282105 (-) 144 WP_003217288.1 YqzL family protein -
  AB0R75_RS11545 (AB0R75_11545) era 2282254..2283159 (-) 906 WP_088000408.1 GTPase Era -
  AB0R75_RS11550 (AB0R75_11550) - 2283152..2283550 (-) 399 WP_144481943.1 cytidine deaminase -
  AB0R75_RS11555 (AB0R75_11555) - 2283664..2284050 (-) 387 WP_144522100.1 diacylglycerol kinase family protein -
  AB0R75_RS11560 (AB0R75_11560) ybeY 2284028..2284507 (-) 480 WP_144522102.1 rRNA maturation RNase YbeY -
  AB0R75_RS11565 (AB0R75_11565) - 2284508..2286595 (-) 2088 WP_186325045.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29230.90 Da        Isoelectric Point: 8.4348

>NTDB_id=1020272 AB0R75_RS11535 WP_012010612.1 2281155..2281925(-) (recO) [Bacillus pumilus strain JJ1622]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=1020272 AB0R75_RS11535 WP_012010612.1 2281155..2281925(-) (recO) [Bacillus pumilus strain JJ1622]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGTGTGATGGCAAGAGGCGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCTTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGTGAAATGATTGAAAGTATGCGGACC
ATTCGGGAAGATTTATTTTTGACCGCATATGCCGCATACATGACAGAGTTACTCGATAAAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCCTGCTTCAATCTCTGCGTCATCTGAATGAAGGCACAGATGCTGATATTATCTTATTTA
TCGTAGAGGTTAAAATGTTATCAGTGATGGGGATGAAGCCCGAACTTGATCAATGTGTTCATTGTGGTCAAAAAGAAGGG
CAGTTTCATTTCTCAATTAGAGATAACGGCTTCATTTGCCAAAATTGCTTTTCTAAAGACCCGTATAAACTGCCCTTATC
CCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTACTATGACGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAAATGGAATCCATGAAAAAGCTCATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8FFB2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754

Multiple sequence alignment