Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AB0R75_RS11265 Genome accession   NZ_CP160232
Coordinates   2232029..2233099 (-) Length   356 a.a.
NCBI ID   WP_211064169.1    Uniprot ID   -
Organism   Bacillus pumilus strain JJ1622     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2227029..2238099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R75_RS11215 (AB0R75_11215) sipW 2227124..2227696 (-) 573 WP_211064171.1 signal peptidase I SipW -
  AB0R75_RS11220 (AB0R75_11220) tapA 2227748..2228272 (-) 525 WP_211064170.1 amyloid fiber anchoring/assembly protein TapA -
  AB0R75_RS11225 (AB0R75_11225) - 2228572..2228850 (+) 279 WP_249407469.1 DUF3889 domain-containing protein -
  AB0R75_RS11230 (AB0R75_11230) - 2228884..2229078 (-) 195 WP_012010560.1 YqzE family protein -
  AB0R75_RS11235 (AB0R75_11235) comGG 2229138..2229521 (-) 384 WP_144522046.1 competence type IV pilus minor pilin ComGG -
  AB0R75_RS11240 (AB0R75_11240) comGF 2229518..2230048 (-) 531 WP_249407460.1 competence type IV pilus minor pilin ComGF -
  AB0R75_RS11245 (AB0R75_11245) comGE 2229957..2230274 (-) 318 WP_144522050.1 competence type IV pilus minor pilin ComGE -
  AB0R75_RS11250 (AB0R75_11250) comGD 2230258..2230704 (-) 447 WP_144522052.1 competence type IV pilus minor pilin ComGD -
  AB0R75_RS11255 (AB0R75_11255) comGC 2230697..2230990 (-) 294 WP_144522054.1 competence type IV pilus major pilin ComGC Machinery gene
  AB0R75_RS11260 (AB0R75_11260) comGB 2231008..2232087 (-) 1080 WP_249407457.1 competence type IV pilus assembly protein ComGB -
  AB0R75_RS11265 (AB0R75_11265) comGA 2232029..2233099 (-) 1071 WP_211064169.1 competence type IV pilus ATPase ComGA Machinery gene
  AB0R75_RS11270 (AB0R75_11270) - 2233277..2234149 (-) 873 WP_211064168.1 STAS domain-containing protein -
  AB0R75_RS11280 (AB0R75_11280) - 2234373..2234753 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AB0R75_RS11285 (AB0R75_11285) - 2234966..2235208 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  AB0R75_RS11290 (AB0R75_11290) - 2235453..2236091 (-) 639 WP_144522058.1 MBL fold metallo-hydrolase -
  AB0R75_RS11295 (AB0R75_11295) - 2236249..2236422 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  AB0R75_RS11300 (AB0R75_11300) - 2236451..2236765 (-) 315 WP_211064167.1 MTH1187 family thiamine-binding protein -
  AB0R75_RS11305 (AB0R75_11305) - 2236844..2238097 (-) 1254 WP_335349181.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40300.76 Da        Isoelectric Point: 9.4258

>NTDB_id=1020271 AB0R75_RS11265 WP_211064169.1 2232029..2233099(-) (comGA) [Bacillus pumilus strain JJ1622]
MYGIENLGQELLEEACRMRASDVHIVPKEKEATVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRRGQVFIRMSTLPTVNNESLVIRILPQDHVPKIKHLSLFPKASTKLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGEICQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1020271 AB0R75_RS11265 WP_211064169.1 2232029..2233099(-) (comGA) [Bacillus pumilus strain JJ1622]
TTGTATGGGATTGAAAATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTTCC
AAAAGAAAAGGAAGCGACGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAAAGAACTATTGATAAAAAAAGTGGCG
AGCGACTCATTGCTCATTTTAAATTTTTATCTTCTATGGATATTGGGGAAAAAAGGAGACCTCAAAATGGGTCACTAGCT
GTGATGCTAAGAAGAGGCCAGGTGTTTATTCGAATGTCTACCTTACCAACTGTAAATAATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCCACCAAATTATTATCGTTTTTGA
ATCATTCGCATGGGCTGATTTTATTTACCGGTCCAACCAATTCAGGGAAAACAACAACCCTCTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGACCCAGTAGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCACGAACGGCAATTAGAGCAGCGCTAACTGGCCATTTAGTGATGAGTACACTCCAT
GCGAAAAATGCAAAAGGAGCTCTTTATCGTATGCTTGAATTTGGTGTCACAATGAATGAACTTGAACAAACAATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAATATGTCAGCTTTACTGTAAATTAAATCGAC
CAGTCAGGCGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCGAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTTGGCTATTTATCGAAAAATACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64

Multiple sequence alignment