Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AB0R87_RS11400 Genome accession   NZ_CP160224
Coordinates   2253723..2254793 (-) Length   356 a.a.
NCBI ID   WP_251190317.1    Uniprot ID   -
Organism   Bacillus pumilus strain JJ950     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2248723..2259793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R87_RS11355 (AB0R87_11355) sipW 2248817..2249389 (-) 573 WP_340866350.1 signal peptidase I SipW -
  AB0R87_RS11360 (AB0R87_11360) tapA 2249441..2249968 (-) 528 WP_335365366.1 amyloid fiber anchoring/assembly protein TapA -
  AB0R87_RS11365 (AB0R87_11365) - 2250268..2250546 (+) 279 WP_003217457.1 DUF3889 domain-containing protein -
  AB0R87_RS11370 (AB0R87_11370) - 2250580..2250774 (-) 195 WP_034620826.1 YqzE family protein -
  AB0R87_RS11375 (AB0R87_11375) comGG 2250834..2251217 (-) 384 WP_003217479.1 competence type IV pilus minor pilin ComGG -
  AB0R87_RS11380 (AB0R87_11380) comGF 2251214..2251969 (-) 756 WP_366595992.1 competence type IV pilus minor pilin ComGF -
  AB0R87_RS11385 (AB0R87_11385) comGD 2251953..2252399 (-) 447 WP_003217424.1 competence type IV pilus minor pilin ComGD -
  AB0R87_RS11390 (AB0R87_11390) comGC 2252392..2252685 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  AB0R87_RS11395 (AB0R87_11395) comGB 2252702..2253742 (-) 1041 WP_366595995.1 competence type IV pilus assembly protein ComGB -
  AB0R87_RS11400 (AB0R87_11400) comGA 2253723..2254793 (-) 1071 WP_251190317.1 competence type IV pilus ATPase ComGA Machinery gene
  AB0R87_RS11405 (AB0R87_11405) - 2254971..2255843 (-) 873 WP_366595997.1 STAS domain-containing protein -
  AB0R87_RS11415 (AB0R87_11415) - 2256067..2256447 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AB0R87_RS11420 (AB0R87_11420) - 2256660..2256902 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  AB0R87_RS11425 (AB0R87_11425) - 2257147..2257785 (-) 639 WP_366595999.1 MBL fold metallo-hydrolase -
  AB0R87_RS11430 (AB0R87_11430) - 2257943..2258116 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  AB0R87_RS11435 (AB0R87_11435) - 2258142..2258456 (-) 315 WP_034662003.1 MTH1187 family thiamine-binding protein -
  AB0R87_RS11440 (AB0R87_11440) - 2258535..2259788 (-) 1254 WP_003217413.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40337.78 Da        Isoelectric Point: 9.5361

>NTDB_id=1019921 AB0R87_RS11400 WP_251190317.1 2253723..2254793(-) (comGA) [Bacillus pumilus strain JJ950]
MYGIEYLGQELLEEACRMRASDVHIVPKEKKASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRRGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECVKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1019921 AB0R87_RS11400 WP_251190317.1 2253723..2254793(-) (comGA) [Bacillus pumilus strain JJ950]
TTGTATGGGATTGAATACTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTTCC
AAAGGAAAAGAAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGAGAAAAACGGAGACCGCAAAATGGGTCATTAGCT
GTGATGCTAAGAAGGGGTCAGGTGTTTATTCGGATGTCTACCCTACCAACTGTAAATGATGAGAGCTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCAAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCCACCAGGTTATTATCATTTTTGA
ATCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCAGGGAAAACAACAACCCTTTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAACAGAAATATTATTACACTTGAAGATCCTGTGGAAACGAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCCGGTATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACACTCCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTGGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGCCAGCTTTACTGTAAATTAAATCGAC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTTGGTGAGTGTGTCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.124

99.438

0.638

Multiple sequence alignment