Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AB0R87_RS05280 Genome accession   NZ_CP160224
Coordinates   1081277..1084969 (+) Length   1230 a.a.
NCBI ID   WP_366597228.1    Uniprot ID   -
Organism   Bacillus pumilus strain JJ950     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1076277..1089969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R87_RS05265 (AB0R87_05265) - 1076570..1076980 (+) 411 WP_106029930.1 DoxX family protein -
  AB0R87_RS05270 (AB0R87_05270) - 1077178..1077624 (-) 447 WP_003211961.1 ferritin-like domain-containing protein -
  AB0R87_RS05275 (AB0R87_05275) addB 1077790..1081299 (+) 3510 WP_366597226.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AB0R87_RS05280 (AB0R87_05280) addA 1081277..1084969 (+) 3693 WP_366597228.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AB0R87_RS05285 (AB0R87_05285) sbcD 1084996..1086171 (+) 1176 WP_044139868.1 exonuclease subunit SbcD -
  AB0R87_RS05290 (AB0R87_05290) sbcC 1086168..1089563 (+) 3396 WP_366597230.1 exonuclease subunit SbcC -
  AB0R87_RS05295 (AB0R87_05295) - 1089583..1089882 (+) 300 WP_366597232.1 HNH endonuclease -

Sequence

Protein


Download         Length: 1230 a.a.        Molecular weight: 142000.06 Da        Isoelectric Point: 5.1209

>NTDB_id=1019899 AB0R87_RS05280 WP_366597228.1 1081277..1084969(+) (addA) [Bacillus pumilus strain JJ950]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALLEKSTDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQPYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDIVLSHPSTFTISFTQASDL
LKEDMSLEKKEQDEIVQALIDGLPVEGYADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKARAT
LYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLESLQQRDLLTDEQIQSINQEGIASFFSTSIGQKL
LKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTVDAAEPILKKRYETQIA
LYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3693 bp        

>NTDB_id=1019899 AB0R87_RS05280 WP_366597228.1 1081277..1084969(+) (addA) [Bacillus pumilus strain JJ950]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCTTCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTTCGTTTGGCTG
ACCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGACGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAATTGGTTGATCGTTACACATCTGACCGTCACGACTTAGATTTGCAATGGCTCGTGAAGAGAATTTATGAGTT
TTCGAGATCTCATCCTTCTCCGGAGCAATGGATGCGGGCATTTCTTTCACTTTATGATGTAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCAGGCGGGCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAGCTTTATGAGCTTTTGCCGAACGTTAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGACCCTGCTCTCTTAGAAAAATCCACTGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGTTTCGAAAGAGCGAAGCAGGAAAAGTCAATTGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCCAAATACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATATCAGGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTATCTAAGGGGAA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCTATTTATCGTTTTAGGCTGGCAGAGCCTATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTGGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACCGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGTAATTTGCAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCTTGGGCTCCACAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCTGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCATTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTATTGTTCATTTAGACGAAAATGAATTGGCTCTGATTCGAACGAGTGA
TAAAAAAGGCACGTATTATGATGCTGTAAAGGCATTCATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAACTAG
AGCGATTTTTCCAATTACTGCGCAAGTGGCGTGATTTTTCTATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGTGATACGCAGTATCTTGATTATGTTGGCGGAATGCCTGGAGGTAAGCAGCGACAGGCAAATCTTCGTGCCCTTTATGA
CCGGGCTAAGCAATACGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGATGATCTTGGTGCAGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGTATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGGCTCGGCAGAAACTTTAATATGATGGACTTAAACCAGCCCTATTTACTTGA
TAAAGAACTAGGCTTTGGAAGCAAGTACATCCATCCTGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTGTTATCTGAAGAATTAAGGGTCCTATATGTAGCGCTCACAAGGGCTAAAGAAAAATTA
TTTCTAGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGGTATCAATCGAAAACATACTTAGATTTCATTGGCCCTGCCCTTATCCGTCATCAAGCGATGTCAT
CTGTTTTAGAAGAAACAGGAGATATTGTTCTTTCGCATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAAAAGGAGCAAGACGAGATTGTACAAGCATTGATCGATGGTCTACCTGTTGAAGG
ATATGCTGATGAGAAGGTGGCAGAGAGACTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAAAGCAGT
CTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGTGCAACA
TTGTATGACCGGCCAGCTTTCATGAAGAAGAAAACACTCACTGCCGCAGAACAAGGCACCGCAATGCATACAGTGATGCA
GCATATCCCGCTCCCTTCTGAGGAGCCCTATGATGAATCACGTATAGGACACCTGCTCGAATCCTTACAGCAGCGAGACT
TGCTAACAGATGAGCAGATCCAATCAATTAATCAAGAAGGAATTGCTTCTTTCTTCTCTACTTCAATTGGTCAAAAACTG
CTAAAAGCGGACTGGGTAAAACGAGAGGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATATTGATAC
TGAGGGAGAGCCTGTTCTGATTCAAGGGATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAGATTATA
AAACTGATAGAGTACAAGGCAGATATACAGTTGACGCTGCTGAACCGATCTTGAAAAAGCGTTATGAAACCCAAATAGCC
TTATATGCAAAAGCTGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTCTATTTCTTCGATGGAAATTTAGA
AATTTCTTTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1050-1222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.37

100

0.667

Multiple sequence alignment