Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AB0R82_RS12890 Genome accession   NZ_CP160220
Coordinates   2511048..2511458 (-) Length   136 a.a.
NCBI ID   WP_198878615.1    Uniprot ID   -
Organism   Bacillus subtilis strain JM553     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2506048..2516458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R82_RS12860 (AB0R82_12860) - 2506135..2506275 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  AB0R82_RS12865 (AB0R82_12865) yqeF 2506581..2507312 (-) 732 WP_029317882.1 SGNH/GDSL hydrolase family protein -
  AB0R82_RS12870 (AB0R82_12870) cwlH 2507564..2508316 (-) 753 WP_128993171.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  AB0R82_RS12875 (AB0R82_12875) yqeD 2508503..2509129 (+) 627 WP_038429330.1 TVP38/TMEM64 family protein -
  AB0R82_RS12880 (AB0R82_12880) gnd 2509148..2510041 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  AB0R82_RS12885 (AB0R82_12885) yqeB 2510293..2511015 (+) 723 WP_010886572.1 hypothetical protein -
  AB0R82_RS12890 (AB0R82_12890) nucA/comI 2511048..2511458 (-) 411 WP_198878615.1 sporulation-specific Dnase NucB Machinery gene
  AB0R82_RS12895 (AB0R82_12895) - 2511654..2512001 (+) 348 Protein_2490 sigma-70 family RNA polymerase sigma factor -
  AB0R82_RS12900 (AB0R82_12900) spoIVCA 2512032..2513492 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  AB0R82_RS12905 (AB0R82_12905) - 2513450..2513628 (-) 179 Protein_2492 hypothetical protein -
  AB0R82_RS12910 (AB0R82_12910) arsC 2513983..2514402 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  AB0R82_RS12915 (AB0R82_12915) acr3 2514414..2515454 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  AB0R82_RS12920 (AB0R82_12920) arsK 2515477..2515917 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  AB0R82_RS12925 (AB0R82_12925) arsR 2515978..2516295 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14968.92 Da        Isoelectric Point: 5.1853

>NTDB_id=1019708 AB0R82_RS12890 WP_198878615.1 2511048..2511458(-) (nucA/comI) [Bacillus subtilis strain JM553]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFNVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1019708 AB0R82_RS12890 WP_198878615.1 2511048..2511458(-) (nucA/comI) [Bacillus subtilis strain JM553]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
ATGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment