Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AB0R90_RS05570 Genome accession   NZ_CP160217
Coordinates   1081645..1085349 (+) Length   1234 a.a.
NCBI ID   WP_099567266.1    Uniprot ID   -
Organism   Bacillus velezensis strain AP52     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1076645..1090349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R90_RS05555 (AB0R90_05555) - 1076726..1077235 (-) 510 WP_007409173.1 ferritin-like domain-containing protein -
  AB0R90_RS05560 (AB0R90_05560) - 1077304..1078017 (-) 714 WP_079978834.1 DUF421 domain-containing protein -
  AB0R90_RS05565 (AB0R90_05565) addB 1078158..1081658 (+) 3501 WP_099566542.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AB0R90_RS05570 (AB0R90_05570) addA 1081645..1085349 (+) 3705 WP_099567266.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AB0R90_RS05575 (AB0R90_05575) sbcD 1085414..1086586 (+) 1173 WP_099566543.1 exonuclease subunit SbcD -
  AB0R90_RS05580 (AB0R90_05580) sbcC 1086583..1089975 (+) 3393 WP_059367267.1 exonuclease subunit SbcC -
  AB0R90_RS05585 (AB0R90_05585) - 1089991..1090284 (+) 294 WP_059367265.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141261.67 Da        Isoelectric Point: 5.1876

>NTDB_id=1019448 AB0R90_RS05570 WP_099567266.1 1081645..1085349(+) (addA) [Bacillus velezensis strain AP52]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELADRYTTDRHDLDLQDLVKRVYEYSRSHPDPEVWLQSFVRLYDVTEESKMEELPFYQYVREDAEMALFGAKQKLEKA
LELTKVPGGPAPRADNFLDDLQQIEELISCRHDFDALYERVPAVSFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKVK
TDYFTRSPQDHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAVDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGAGTGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADGADSSEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANADRHDELYQKLRTFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHRDMEAHRTPGLSSSEDIARDPSRFHIRMLQQSE
LLEENPKERAEEKSKRLKAIQQGEPIPDSFSFDDQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYRRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRMLYEKELLTEEQQEAIDIEEIVQFFGTEIGK
DLLRALRVDREVPFSMALPAGEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAVEQITKTKVKGRALYFFDGGHVLTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=1019448 AB0R90_RS05570 WP_099567266.1 1081645..1085349(+) (addA) [Bacillus velezensis strain AP52]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGAAGAAGATC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAGATGAAGCATCGGATCGCGGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTGCATATCAGACGGCAGCTCTCTCTCTTGAATCGGGCGAGCATTTC
GACGCTTCACTCTTTCTGTCTGCAAGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCCGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTTCTTGATGAGCTGTTTGAAGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGCGGACAGATATACGACAGACAGGCATGATCTTGATCTTCAAGACCTTGTCAAACGGGTGTACGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGTATGGCTGCAAAGCTTTGTCCGCCTGTATGATGTGACGGAAGAAAGCAAAATGG
AAGAGCTTCCGTTTTACCAATATGTGAGAGAAGATGCTGAAATGGCGCTTTTCGGGGCAAAACAAAAGCTTGAGAAGGCG
CTTGAACTGACAAAAGTGCCCGGCGGGCCGGCCCCGCGCGCTGATAATTTTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTCGATGCGCTGTATGAACGGGTTCCCGCAGTCTCCTTTAAACGGGCGAAGGCGGTAAAGG
GTGATGAATTTGACAAAGCGCTTTTGGACGAAGCGACCGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGGTAAAG
ACCGATTATTTTACAAGAAGTCCGCAAGATCACTTAAAGAGTTTAGCAGATATGAAGCCTGTCATCGAAACGCTCGTTCA
GCTCGTGATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATTATTGATTTCTCAGATTTGGAACATT
ACTGCCTGGCGATTTTAACTGCGGTTGATGAAGAAGGCCGGCGGGTGCCGAGCGAAGCGGCCGTTTATTATCAAGACCAG
TTCCATGAAGTTCTGGTTGATGAATATCAAGATACGAATCTTGTGCAAGAGTCTATCCTGCAGCTTGTTAAAAGCGGGAA
TGAAGAGGCGGGAAATCTGTTTATGGTAGGCGACGTCAAACAATCGATCTACCGTTTCAGACTTGCTGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAAAGCGGGGCAGGCACGGGGCAGAAAATTGACCTGAACCAAAATTTCCGCAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGAAAAATCGGCGAAGTGGATTACGATGA
ACAGGCGGCATTGAAGCTCGGCGCATCGTATCCGCCGAATGACGCGGCAAAAACGGAACTGCTGCTCATTGACAGCGCTG
ATGGCGCAGACAGCTCCGAGGATGCCGAAGACTTTGAAACTGTGCACTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGA
AAGCTTGTCTCATCTCCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTAAT
TTTGCTGCGCTCCATGCCGTGGGCGCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTGTACGCCAATTTGA
CGTCGGGTTACTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTGAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTTGCTTCCGTGCTGCGCTCGCCCATCGTCGGCTGTGACGAAAATGAACTTGCGCTGATACGCCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCATATATAGCGAACGCTGACAGACATGATGAACTGTATCAAAAACTGAGAACCT
TTTATGACAGTCTGCAAAAGTGGCGGTCATTTTCGACGAATCATTCGGTGTCTGAGCTGATTTGGGAAGTATACCGCGAT
ACCGGATATTTTGACTATGCAGGCGGCATGCCCGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGCTCGTACGAAGCGACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATCTGGGGACGGCCCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
GCTCGGTTTCGGCACGAAGTATATCCATCCGGAACTCAGAATCAGCTATCCGACTCTTCCGCTTGTGGCGATGAAGAAAA
AGATGCGCAGAGAGCTCTTATCCGAAGAATTGCGCGTACTGTATGTGGCGCTGACCAGAGCGAAGGAAAAGCTGTTTCTC
GTCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCCGATTGGCTTCTTTCTGA
ATTCGACCGCTATCAGGCGTCATCTTATTTGGACTTTATCGGGCCGGCGCTCATCCGCCACCGTGACATGGAGGCGCACA
GGACGCCGGGACTTTCCTCATCTGAGGATATCGCGCGCGACCCGTCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAA
TTGCTGGAAGAGAATCCAAAAGAGCGTGCTGAAGAAAAGAGCAAACGTCTAAAAGCAATACAGCAGGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTGCTTGAATGGGAATATCCGTACCGAGAGCTGACGGCTATCAGAA
CGAAGCAATCAGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCTCTCATCAAACCGTCA
GGAGACACGCTTTTATACAGGCGTCCCGGTTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCACGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTCCGGATGCTTTACGAAA
AAGAGCTGCTGACAGAAGAACAGCAGGAAGCTATTGATATAGAAGAAATTGTCCAGTTTTTCGGAACTGAAATCGGGAAA
GACCTTCTCAGGGCGCTGCGGGTTGACCGGGAAGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTGCAGGGGATTATTGACTGCCTCTACGAAACTGCGGACGGTCTCTATTTGCTGG
ACTATAAAACAGATCGGATAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCTCCGATTCTGCAAAAAAGGTATGAA
ACACAAATTGAATTGTATACAAAAGCGGTTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

82.334

100

0.823

Multiple sequence alignment