Detailed information NTG1018935

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: DQL69_RS00340
• Genome accession: NZ_LS483345
• Coordinates: 54255..55253 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes strain NCTC8231
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49255..60253

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL69_RS09330	-	50034..51363 (+)	1330	Protein_30	hypothetical protein	-
DQL69_RS00330 (NCTC8231_00066)	purB	51732..53024 (+)	1293	WP_023612869.1	adenylosuccinate lyase	-
DQL69_RS00335 (NCTC8231_00067)	comR	53124..54035 (+)	912	WP_014635220.1	helix-turn-helix domain-containing protein	Regulator
DQL69_RS09185 (NCTC8231_00068)	comS	54126..54224 (+)	99	WP_020833188.1	quorum-sensing system DWW-type pheromone	Regulator
DQL69_RS00340 (NCTC8231_00069)	ruvB	54255..55253 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
DQL69_RS00345 (NCTC8231_00070)	-	55391..55828 (+)	438	WP_002994668.1	low molecular weight protein-tyrosine-phosphatase	-
DQL69_RS00350 (NCTC8231_00071)	-	55851..56252 (+)	402	WP_011017240.1	MORN repeat-containing protein	-
DQL69_RS00355 (NCTC8231_00072)	-	56249..58024 (+)	1776	WP_002987732.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=1018935 DQL69_RS00340 WP_002986679.1 54255..55253(+) (ruvB) [Streptococcus pyogenes strain NCTC8231]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=1018935 DQL69_RS00340 WP_002986679.1 54255..55253(+) (ruvB) [Streptococcus pyogenes strain NCTC8231]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCGGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTGCTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGACTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGGCCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACTCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473