Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB0U95_RS03720 Genome accession   NZ_CP160163
Coordinates   773043..774023 (+) Length   326 a.a.
NCBI ID   WP_001055639.1    Uniprot ID   A0A8E6VVQ5
Organism   Salmonella enterica subsp. enterica strain N23-2656     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 768043..779023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0U95_RS03690 (AB0U95_03690) - 768621..769628 (+) 1008 WP_079983848.1 DUF1202 domain-containing protein -
  AB0U95_RS03695 (AB0U95_03695) hemW 769719..770855 (-) 1137 WP_001096532.1 radical SAM family heme chaperone HemW -
  AB0U95_RS03700 (AB0U95_03700) - 770848..771441 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  AB0U95_RS03705 (AB0U95_03705) yggU 771449..771739 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  AB0U95_RS03710 (AB0U95_03710) - 771736..772302 (-) 567 WP_368372218.1 YggT family protein -
  AB0U95_RS03715 (AB0U95_03715) - 772321..773025 (-) 705 WP_058650237.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB0U95_RS03720 (AB0U95_03720) pilT 773043..774023 (+) 981 WP_001055639.1 type IV pilus twitching motility protein PilT Machinery gene
  AB0U95_RS03725 (AB0U95_03725) - 774155..774841 (+) 687 WP_000098337.1 IclR family transcriptional regulator -
  AB0U95_RS03730 (AB0U95_03730) ruvX 774888..775304 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  AB0U95_RS03735 (AB0U95_03735) - 775304..775867 (-) 564 WP_001053171.1 YqgE/AlgH family protein -
  AB0U95_RS03740 (AB0U95_03740) gshB 776083..777030 (-) 948 WP_000593248.1 glutathione synthase -
  AB0U95_RS03745 (AB0U95_03745) rsmE 777050..777781 (-) 732 WP_058331719.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB0U95_RS03750 (AB0U95_03750) endA 777858..778565 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36150.37 Da        Isoelectric Point: 7.5116

>NTDB_id=1018514 AB0U95_RS03720 WP_001055639.1 773043..774023(+) (pilT) [Salmonella enterica subsp. enterica strain N23-2656]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1018514 AB0U95_RS03720 WP_001055639.1 773043..774023(+) (pilT) [Salmonella enterica subsp. enterica strain N23-2656]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCAGCGTTACGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCTGTACTATCACAAAAACTGGAGGT
CGACAGGCAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.637

100

0.374

Multiple sequence alignment