Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ABWT76_RS03260 Genome accession   NZ_CP159837
Coordinates   802218..804236 (+) Length   672 a.a.
NCBI ID   WP_054468852.1    Uniprot ID   -
Organism   Planktothricoides raciborskii GIHE-MW2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 797218..809236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWT76_RS03250 (ABWT76_000646) dnaK 798388..800292 (-) 1905 WP_190878600.1 molecular chaperone DnaK -
  ABWT76_RS03255 (ABWT76_000647) grpE 800438..801238 (-) 801 WP_082348982.1 nucleotide exchange factor GrpE -
  ABWT76_RS03260 (ABWT76_000648) pilB/pilB1 802218..804236 (+) 2019 WP_054468852.1 GspE/PulE family protein Machinery gene
  ABWT76_RS03265 (ABWT76_000649) - 804350..805453 (+) 1104 WP_054468850.1 type IV pilus twitching motility protein PilT -
  ABWT76_RS03270 (ABWT76_000650) - 806394..808061 (+) 1668 WP_354635623.1 DUF1565 domain-containing protein -

Sequence

Protein


Download         Length: 672 a.a.        Molecular weight: 74905.78 Da        Isoelectric Point: 5.3985

>NTDB_id=1016108 ABWT76_RS03260 WP_054468852.1 802218..804236(+) (pilB/pilB1) [Planktothricoides raciborskii GIHE-MW2]
MTYASQRQALVVQSNFSPFGNKLLSSGYVDHNQMKEAIKQSRDMKKPLLEVLQELTDKPLPPELLRQYKVQQLFELKLIY
AIEAFDPELTEISQEQLIDLIDNLLPIETCRLHRILPLYKTETNPAALVVGMVDPDKLDAQDDLNRILKSHSLGFQRRVI
TLEDYQHFLNQYIEEKAQEEAARKKQAQDKANQVDLTVDPNAFADVDIQEADAEDQDDMDLNKSLHAAESAPIVKTVNQI
LAKALSDGVSDIHVEPQEEFLRIRMRKDGVLQEIYRLPSKVVNPLVARFKIIADLDIAERRNAQDGRIRRIFQGRTVDFR
VNVLPSRFGEKICMRILDNSSTQLGLDMLISDPEALAKVREFASRPFGMILVTGPTGSGKSTSLYSVLAERNSPEVNIST
AEDPIEYALPGITQCQVIRAKGLDFNALLRAFMRQDPDIILVGETRDHETAKAAMEAALTGHLVLTTLHTNDAAGAIARL
DEMGIEPFMVAGALIGISAQRLMRRVCSNCKVPYQPSPEDLARYGLSAAKESGVIFHKANFIAPSDRKAAEEAGTLCPKC
RGGGYKGRVGVYEVLKNSDEISALITQRAPTALIKERAVQEGMVSLLPYSLNLVREGHTTFEEVDRVTFTDTGLEAELKA
KRKTGLTCQGCGAGLQPEWLDCPYCMTPRFKD

Nucleotide


Download         Length: 2019 bp        

>NTDB_id=1016108 ABWT76_RS03260 WP_054468852.1 802218..804236(+) (pilB/pilB1) [Planktothricoides raciborskii GIHE-MW2]
ATGACTTACGCATCCCAACGACAGGCTCTTGTCGTCCAAAGTAATTTTTCTCCCTTTGGGAATAAGCTTCTTTCATCCGG
TTATGTGGATCATAACCAGATGAAAGAAGCAATTAAACAAAGCCGGGACATGAAAAAGCCCCTGCTAGAAGTTCTCCAAG
AACTGACAGACAAACCACTGCCCCCGGAATTACTGCGCCAGTACAAAGTGCAGCAACTCTTTGAACTGAAGCTGATTTAT
GCGATCGAAGCCTTCGATCCCGAACTAACCGAAATTAGCCAAGAACAGCTAATTGATCTGATTGACAATTTGCTGCCCAT
TGAAACCTGTCGCCTCCATCGCATTCTGCCGCTATATAAAACCGAAACGAATCCCGCAGCTTTGGTCGTAGGGATGGTCG
ATCCAGACAAGTTGGATGCACAGGATGACCTCAACCGAATCTTGAAATCCCATTCCCTGGGATTCCAGCGTCGGGTAATT
ACCCTCGAAGATTATCAACACTTCCTGAATCAATACATAGAAGAAAAAGCGCAGGAAGAAGCCGCCCGGAAAAAGCAGGC
GCAAGATAAAGCCAACCAAGTCGATCTCACCGTCGATCCGAATGCCTTCGCCGATGTGGATATTCAGGAAGCTGACGCTG
AGGATCAAGATGACATGGACCTCAACAAGTCTCTGCACGCAGCCGAGTCAGCGCCGATTGTGAAGACCGTGAACCAAATC
CTGGCTAAAGCCTTGAGCGATGGGGTATCGGACATCCACGTCGAACCCCAAGAAGAATTTCTCCGGATCCGGATGCGGAA
AGACGGGGTACTCCAAGAAATCTATAGGCTGCCCTCAAAAGTGGTCAACCCCTTGGTGGCTCGCTTTAAAATTATTGCCG
ACCTGGACATTGCCGAACGCCGGAATGCTCAAGACGGTCGGATCCGCCGGATCTTTCAGGGACGCACGGTGGATTTCCGG
GTGAATGTTCTGCCCAGCCGATTTGGGGAAAAAATCTGTATGCGGATTTTGGATAACTCCTCCACCCAACTCGGTTTGGA
TATGTTAATCAGCGATCCGGAAGCTTTGGCCAAAGTGCGGGAGTTCGCCAGTCGTCCTTTTGGCATGATTTTGGTGACGG
GGCCGACGGGTTCTGGGAAGTCCACCTCTTTGTATTCGGTGTTAGCGGAACGCAACTCCCCAGAGGTGAATATTTCCACG
GCAGAAGACCCGATTGAGTATGCGTTGCCGGGGATTACCCAATGTCAGGTGATTCGGGCGAAGGGTTTGGATTTTAACGC
GCTGTTGCGGGCGTTTATGCGCCAAGACCCGGATATTATTCTGGTGGGGGAAACGCGGGACCATGAAACGGCCAAAGCAG
CTATGGAAGCGGCGTTAACCGGCCACTTAGTGTTAACCACTCTGCATACTAACGACGCCGCAGGAGCGATCGCCCGTTTA
GATGAAATGGGCATTGAACCCTTCATGGTGGCAGGGGCGCTGATTGGCATTTCCGCACAGCGCTTAATGCGTCGGGTTTG
CAGCAATTGCAAAGTTCCCTATCAACCCAGCCCAGAAGATTTGGCTCGATATGGTTTATCCGCCGCCAAAGAATCCGGGG
TGATATTCCACAAAGCCAATTTTATTGCTCCGAGCGATCGAAAAGCCGCCGAAGAAGCGGGCACCCTTTGTCCCAAATGT
CGCGGTGGTGGCTACAAAGGTCGGGTTGGCGTCTATGAAGTGCTGAAAAACAGTGATGAAATCTCGGCCTTAATCACTCA
ACGGGCTCCGACCGCTTTGATTAAAGAAAGAGCAGTCCAAGAAGGGATGGTATCCCTACTGCCCTATAGCCTCAATCTCG
TGCGGGAAGGCCATACAACTTTTGAAGAAGTAGACCGGGTGACTTTTACCGATACGGGCTTAGAGGCGGAACTCAAAGCC
AAGCGGAAAACAGGTCTGACCTGTCAGGGTTGTGGGGCGGGTTTACAGCCAGAATGGTTAGATTGTCCCTACTGCATGAC
CCCTCGCTTTAAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(239-505)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(70-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

60

98.958

0.594

Multiple sequence alignment