Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   JMW19_RS21100 Genome accession   NZ_LR890714
Coordinates   4430522..4431904 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain MSB1_6C isolate MSB1_6C     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4425522..4436904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMW19_RS21070 - 4426170..4427180 (+) 1011 WP_000023622.1 aldo/keto reductase -
  JMW19_RS21075 - 4427200..4427997 (+) 798 WP_001550574.1 SDR family oxidoreductase -
  JMW19_RS21080 - 4428023..4428439 (+) 417 WP_001140838.1 SRPBCC family protein -
  JMW19_RS21085 - 4428597..4428809 (+) 213 WP_000848792.1 DUF1471 domain-containing protein -
  JMW19_RS25910 - 4428882..4428998 (+) 117 Protein_4194 hypothetical protein -
  JMW19_RS21095 nadR 4429269..4430501 (-) 1233 WP_000093813.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  JMW19_RS21100 radA/sms 4430522..4431904 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  JMW19_RS21105 serB 4431953..4432921 (-) 969 WP_001132956.1 phosphoserine phosphatase -
  JMW19_RS21110 ytjB 4433027..4433671 (+) 645 WP_001550573.1 YtjB family periplasmic protein -
  JMW19_RS21115 lplA 4433699..4434715 (+) 1017 WP_001550572.1 lipoate--protein ligase LplA -
  JMW19_RS21120 - 4434747..4435010 (+) 264 WP_000566139.1 helix-turn-helix transcriptional regulator -
  JMW19_RS21125 deoD 4435171..4435890 (-) 720 WP_000224877.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=1015079 JMW19_RS21100 WP_001029698.1 4430522..4431904(-) (radA/sms) [Escherichia coli strain MSB1_6C isolate MSB1_6C]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=1015079 JMW19_RS21100 WP_001029698.1 4430522..4431904(-) (radA/sms) [Escherichia coli strain MSB1_6C isolate MSB1_6C]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGCGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCAGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGTGGCGTGGTGCCGGGAAGTGCCATTCTGATTGGCGGTAACCCAGGTGCCGG
GAAATCCACATTGCTATTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACTGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACCGACAATCTCAATATGTTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGT
TCAGTCATCGCCTGGCAGTGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGCGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCTGCCATCTTTTTAAGCCGCGGTGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTAGTGTGGGAAGGAACGCGTCCGCTGCTGGTGGAGATCCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTCGCAGTAGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCGGTGCTGCACCG
TCACGGTGGGTTGCAAATGGCCGATCAGGATGTATTTGTTAACGTGGTCGGCGGCGTGAAGGTGACGGAAACCAGCGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGCCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAATGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43