Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   APECO18_RS13135 Genome accession   NZ_CP006830
Coordinates   2590655..2591332 (+) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli APEC O18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2585655..2596332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APECO18_RS13100 (APECO18_12775) gspG 2586117..2586554 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  APECO18_RS13105 (APECO18_12780) gspH 2586562..2587071 (+) 510 WP_001076038.1 type II secretion system minor pseudopilin GspH -
  APECO18_RS13110 (APECO18_12785) gspI 2587068..2587463 (+) 396 WP_001041747.1 type II secretion system minor pseudopilin GspI -
  APECO18_RS13115 (APECO18_12790) gspJ 2587456..2588043 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  APECO18_RS13120 (APECO18_12795) gspK 2588036..2589019 (+) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  APECO18_RS13125 (APECO18_12800) gspL 2589034..2590197 (+) 1164 WP_001332436.1 type II secretion system protein GspL -
  APECO18_RS13130 (APECO18_12805) gspM 2590194..2590655 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  APECO18_RS13135 (APECO18_12810) pilD 2590655..2591332 (+) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  APECO18_RS13140 (APECO18_12815) bfr 2591361..2591837 (-) 477 WP_000675504.1 bacterioferritin -
  APECO18_RS13145 (APECO18_12820) bfd 2591909..2592103 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  APECO18_RS13150 (APECO18_12825) chiA 2592272..2594974 (-) 2703 WP_000773166.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=101496 APECO18_RS13135 WP_000178175.1 2590655..2591332(+) (pilD) [Escherichia coli APEC O18]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=101496 APECO18_RS13135 WP_000178175.1 2590655..2591332(+) (pilD) [Escherichia coli APEC O18]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387

Multiple sequence alignment