Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   JMW69_RS19600 Genome accession   NZ_LR890606
Coordinates   4080492..4081877 (-) Length   461 a.a.
NCBI ID   WP_069905174.1    Uniprot ID   -
Organism   Escherichia coli strain MINF_8D isolate MINF_8D     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4075492..4086877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMW69_RS19585 ettA 4077267..4078934 (+) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -
  JMW69_RS19590 nadS 4079095..4079208 (-) 114 Protein_3889 NadS family protein -
  JMW69_RS19595 nadR 4079242..4080474 (-) 1233 WP_000093809.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  JMW69_RS19600 radA/sms 4080492..4081877 (-) 1386 WP_069905174.1 DNA repair protein RadA Machinery gene
  JMW69_RS19605 serB 4081926..4082894 (-) 969 WP_001132956.1 phosphoserine phosphatase -
  JMW69_RS19610 ytjB 4083000..4083644 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  JMW69_RS19615 lplA 4083672..4084688 (+) 1017 WP_000105848.1 lipoate--protein ligase LplA -
  JMW69_RS25830 - 4084720..4084983 (+) 264 Protein_3895 helix-turn-helix transcriptional regulator -
  JMW69_RS19625 deoD 4085144..4085863 (-) 720 WP_000224877.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 49555.18 Da        Isoelectric Point: 7.1936

>NTDB_id=1014729 JMW69_RS19600 WP_069905174.1 4080492..4081877(-) (radA/sms) [Escherichia coli strain MINF_8D isolate MINF_8D]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDLL

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1014729 JMW69_RS19600 WP_069905174.1 4080492..4081877(-) (radA/sms) [Escherichia coli strain MINF_8D isolate MINF_8D]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGACATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGCGGCGTGGTACCGGGAAGTGCCATTCTGATTGGCGGTAACCCAGGCGCGGG
GAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGT
ACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTGGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCCGCCATCTTTTTAAGCCGTGGCGATGAAG
TGACCTCTGGTAGTTCAGTGATGGTGGTATGGGAAGGAACACGTCCGTTGCTGGTGGAGATCCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCGCGCCGCGTGGCGGTAGGTCTGGAGCAAAACCGTCTGGCGATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACGGAAACCAGCGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATTATGA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.132

0.464
  radA Streptococcus mitis NCTC 12261

43.421

98.915

0.43
  radA Streptococcus pneumoniae Rx1

43.421

98.915

0.43
  radA Streptococcus pneumoniae D39

43.421

98.915

0.43
  radA Streptococcus pneumoniae R6

43.421

98.915

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

98.915

0.43
  radA Streptococcus mitis SK321

43.421

98.915

0.43