Detailed information NTG1014650

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABV836_RS14500
• Genome accession: NZ_CP159579
• Coordinates: 3018636..3019754 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain Ab6-Co-2
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3013636..3024754

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABV836_RS14490 (ABV836_14490)	-	3016752..3017444 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABV836_RS14495 (ABV836_14495)	pilT	3017571..3018608 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ABV836_RS14500 (ABV836_14500)	pilU	3018636..3019754 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABV836_RS14505 (ABV836_14505)	fur	3019824..3020261 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ABV836_RS14510 (ABV836_14510)	bamE	3020373..3020771 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ABV836_RS14515 (ABV836_14515)	-	3020826..3021149 (-)	324	WP_000442587.1	RnfH family protein	-
ABV836_RS14520 (ABV836_14520)	-	3021149..3022216 (-)	1068	WP_002019572.1	hypothetical protein	-
ABV836_RS14525 (ABV836_14525)	-	3022369..3022824 (-)	456	WP_000782976.1	bacteriohemerythrin	-
ABV836_RS14530 (ABV836_14530)	-	3023077..3023925 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=1014650 ABV836_RS14500 WP_000347039.1 3018636..3019754(+) (pilU) [Acinetobacter baumannii strain Ab6-Co-2]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1014650 ABV836_RS14500 WP_000347039.1 3018636..3019754(+) (pilU) [Acinetobacter baumannii strain Ab6-Co-2]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395