Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   JMW03_RS20785 Genome accession   NZ_LR890349
Coordinates   4281590..4282972 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain MSB1_9D isolate MSB1_9D     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4276590..4287972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMW03_RS20775 ettA 4278363..4280030 (+) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -
  JMW03_RS26195 - 4280191..4280286 (-) 96 Protein_4125 hypothetical protein -
  JMW03_RS20780 nadR 4280337..4281569 (-) 1233 WP_000093810.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  JMW03_RS20785 radA/sms 4281590..4282972 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  JMW03_RS20790 serB 4283021..4283989 (-) 969 WP_001132955.1 phosphoserine phosphatase -
  JMW03_RS20795 ytjB 4284095..4284739 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  JMW03_RS20800 lplA 4284767..4285783 (+) 1017 WP_097751165.1 lipoate--protein ligase LplA -
  JMW03_RS20805 - 4285815..4286078 (+) 264 WP_000566150.1 helix-turn-helix transcriptional regulator -
  JMW03_RS20810 deoD 4286238..4286957 (-) 720 WP_000224879.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=1014145 JMW03_RS20785 WP_001029698.1 4281590..4282972(-) (radA/sms) [Escherichia coli strain MSB1_9D isolate MSB1_9D]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=1014145 JMW03_RS20785 WP_001029698.1 4281590..4282972(-) (radA/sms) [Escherichia coli strain MSB1_9D isolate MSB1_9D]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
ACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTGGATGGTGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAG
TGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTATTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCG
ATTTAGCTTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43