Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PPH94_RS15460 Genome accession   NZ_CP159409
Coordinates   3275107..3276612 (+) Length   501 a.a.
NCBI ID   WP_042977154.1    Uniprot ID   -
Organism   Burkholderia cepacia strain SCCH90 Bcn202840     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3270107..3281612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPH94_RS15435 (PPH94_015435) kdsD 3271339..3272322 (+) 984 WP_272546133.1 arabinose 5-phosphate isomerase KdsD -
  PPH94_RS15440 (PPH94_015440) - 3272322..3272858 (+) 537 WP_272546132.1 HAD family hydrolase -
  PPH94_RS15445 (PPH94_015445) lptC 3272862..3273476 (+) 615 WP_027789105.1 LPS export ABC transporter periplasmic protein LptC -
  PPH94_RS15450 (PPH94_015450) lptA 3273497..3274162 (+) 666 WP_021162598.1 lipopolysaccharide transport periplasmic protein LptA -
  PPH94_RS15455 (PPH94_015455) lptB 3274159..3274935 (+) 777 WP_021162599.1 LPS export ABC transporter ATP-binding protein -
  PPH94_RS15460 (PPH94_015460) rpoN/rpoN1 3275107..3276612 (+) 1506 WP_042977154.1 RNA polymerase factor sigma-54 Machinery gene
  PPH94_RS15465 (PPH94_015465) raiA 3276771..3277130 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  PPH94_RS15470 (PPH94_015470) - 3277299..3277838 (+) 540 WP_034195946.1 PTS sugar transporter subunit IIA -
  PPH94_RS15475 (PPH94_015475) hprK 3277970..3278938 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  PPH94_RS15480 (PPH94_015480) rapZ 3279014..3279922 (+) 909 WP_021162602.1 RNase adapter RapZ -
  PPH94_RS15485 (PPH94_015485) - 3279950..3280585 (+) 636 WP_027789102.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54827.96 Da        Isoelectric Point: 4.8767

>NTDB_id=1013493 PPH94_RS15460 WP_042977154.1 3275107..3276612(+) (rpoN/rpoN1) [Burkholderia cepacia strain SCCH90 Bcn202840]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNSSSSSSERAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1013493 PPH94_RS15460 WP_042977154.1 3275107..3276612(+) (rpoN/rpoN1) [Burkholderia cepacia strain SCCH90 Bcn202840]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TAGCGAGCCCGCTGCGTGTCGCAGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGCCCAGAACACCGAGCCCGCTGCG
ACAAACAGCAGCAGTTCGTCGAGCGAGCGCGCCGAGCGCGACGAACCGGCCGGCGCGGACGAATACGACGGTTACGCGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGCGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCACTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCAAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.245

100

0.691

Multiple sequence alignment