Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ABU998_RS13245 Genome accession   NZ_CP159108
Coordinates   2723825..2725195 (+) Length   456 a.a.
NCBI ID   WP_002356050.1    Uniprot ID   -
Organism   Enterococcus faecalis strain 10033598-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2718825..2730195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU998_RS13220 (ABU998_13220) - 2719315..2719782 (-) 468 WP_002408153.1 VOC family protein -
  ABU998_RS13225 (ABU998_13225) - 2719847..2720794 (+) 948 WP_002389777.1 YafY family protein -
  ABU998_RS13230 (ABU998_13230) - 2720886..2722139 (-) 1254 WP_025186428.1 glutamate-5-semialdehyde dehydrogenase -
  ABU998_RS13235 (ABU998_13235) proB 2722126..2722941 (-) 816 WP_002389550.1 glutamate 5-kinase -
  ABU998_RS13240 (ABU998_13240) - 2723261..2723746 (+) 486 WP_002361472.1 dUTP diphosphatase -
  ABU998_RS13245 (ABU998_13245) radA 2723825..2725195 (+) 1371 WP_002356050.1 DNA repair protein RadA Machinery gene
  ABU998_RS13250 (ABU998_13250) - 2725296..2726441 (+) 1146 WP_002368622.1 PIN/TRAM domain-containing protein -
  ABU998_RS13255 (ABU998_13255) ispF 2726460..2726933 (+) 474 WP_002356052.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ABU998_RS13260 (ABU998_13260) gltX 2726986..2728443 (+) 1458 WP_010709111.1 glutamate--tRNA ligase -
  ABU998_RS13265 (ABU998_13265) epsC 2728713..2729252 (+) 540 WP_002356054.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49757.28 Da        Isoelectric Point: 7.3489

>NTDB_id=1012218 ABU998_RS13245 WP_002356050.1 2723825..2725195(+) (radA) [Enterococcus faecalis strain 10033598-1]
MAKKAKVQFECQSCGYVSPKYLGRCPNCGQWNSMVEEVIQDTSDRRARVSLTGKKTQPQRLSEVVPKKEPRVKTELVELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRAERLGTVNETFYLYAETDMHEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMQTHGLVEVMNPSQVFLEERLEGATGSSIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLALAISIASSYKEKGTSSSECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKVYVPKNNLGGWEAPEGIEIIGVSTIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1012218 ABU998_RS13245 WP_002356050.1 2723825..2725195(+) (radA) [Enterococcus faecalis strain 10033598-1]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAAGCTGTGGGTATGTGTCTCCAAAATATCTCGGCCGCTGTCCTAA
TTGTGGACAATGGAATTCGATGGTGGAAGAGGTTATTCAAGATACTTCTGATCGTCGTGCTAGAGTAAGTTTGACTGGTA
AAAAGACACAACCACAACGGCTATCTGAAGTCGTACCTAAAAAAGAGCCTCGTGTAAAAACTGAATTAGTGGAGCTGAAT
CGGGTTTTAGGTGGTGGCGTTGTTCCAGGTTCGTTAGTTTTAATCGGTGGCGATCCCGGAATAGGTAAGTCAACATTGCT
CTTACAAGTATCGCAACAATTAGCAGCCACAGGTGGCACGGTTTTATATGTTTCTGGTGAAGAAAGTGCTGAGCAAATTA
AATTGCGAGCGGAGCGTTTAGGCACCGTCAACGAGACATTTTACTTATACGCAGAAACAGATATGCACGAAATATCACGA
GCCATTGAAAAATTAGAACCAGACTATGTCATTATTGACTCGATTCAAACAATGACGCAGCCAGATGTTACCAGCGTTGC
TGGTAGTGTCAGTCAAGTCCGAGAAACAACTGCCGAATTATTGAAACTGGCAAAAACGAATGGGATTGCCATTTTTATTG
TCGGACACGTAACGAAGGAAGGGTCTATCGCAGGGCCACGGATGTTGGAACATATGGTAGATACGGTTCTTTATTTTGAA
GGAGATAAGCATCACACCTTTAGAATTTTACGGGCTGTCAAAAATCGGTTTGGCTCAACGAATGAGATTGGTATTTTTGA
AATGCAGACACATGGATTGGTTGAAGTTATGAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGAGCCACCGGAT
CTTCAATTGTGGTAGCAATGGAAGGTTCACGCCCTATTTTGGTGGAAATCCAAGCGTTAGTTACCCCAACGATGTTTGGC
AATGCCAAGCGAACGACCACCGGGTTGGACTTTAATCGCGTTTCTTTAATCATGGCGGTTTTAGAAAAACGGGCAGGCCT
TTTGTTACAAAACCAGGATGCGTATCTAAAAGCGGCTGGTGGAGTCAAAATTAATGAACCAGCTATTGATTTAGCGTTAG
CAATCAGTATTGCTTCTAGCTATAAAGAAAAAGGAACCTCATCTTCGGAATGTTTCATTGGTGAGATTGGTTTAACTGGT
GAAATCCGCCGCGTGAATAGTATTGAACAACGAGTGCGTGAAGCGCAAAAACTAGGCTTTACAAAAGTCTATGTCCCTAA
AAATAACCTTGGTGGCTGGGAAGCGCCTGAAGGGATTGAAATTATTGGCGTTTCAACAATTGGTGAAACGTTAAGAAAAG
TTTTTAAATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

75.385

99.781

0.752
  radA Streptococcus pneumoniae Rx1

75.385

99.781

0.752
  radA Streptococcus pneumoniae D39

75.385

99.781

0.752
  radA Streptococcus pneumoniae R6

75.385

99.781

0.752
  radA Streptococcus pneumoniae TIGR4

75.385

99.781

0.752
  radA Streptococcus mitis SK321

75.385

99.781

0.752
  radA Bacillus subtilis subsp. subtilis str. 168

66.297

98.904

0.656

Multiple sequence alignment