Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   H1W81_RS04975 Genome accession   NZ_LR824002
Coordinates   958658..960868 (+) Length   736 a.a.
NCBI ID   WP_011226133.1    Uniprot ID   Q5M3Y5
Organism   Streptococcus thermophilus isolate STH_CIRM_67     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 953658..965868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W81_RS04955 (STHERMO_1085) hflX 955046..956284 (+) 1239 WP_011226137.1 GTPase HflX -
  H1W81_RS04960 (STHERMO_1086) - 956277..956918 (+) 642 WP_011226136.1 cystathionine beta-lyase -
  H1W81_RS04965 (STHERMO_1087) rnz 956949..957878 (+) 930 WP_011226135.1 ribonuclease Z -
  H1W81_RS04970 (STHERMO_1088) - 957878..958645 (+) 768 WP_011226134.1 SDR family NAD(P)-dependent oxidoreductase -
  H1W81_RS04975 (STHERMO_1089) recJ 958658..960868 (+) 2211 WP_011226133.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  H1W81_RS04980 (STHERMO_1090) - 960983..961501 (+) 519 WP_002947203.1 adenine phosphoribosyltransferase -
  H1W81_RS04985 (STHERMO_1091) metA 961672..962616 (+) 945 WP_011226132.1 homoserine O-acetyltransferase MetA -
  H1W81_RS04990 (STHERMO_1092) - 962688..963386 (+) 699 WP_002947201.1 DnaD domain-containing protein -
  H1W81_RS04995 (STHERMO_1093) nth 963386..964030 (+) 645 WP_011681255.1 endonuclease III -
  H1W81_RS05000 (STHERMO_1096) - 964663..964908 (+) 246 WP_011226130.1 hypothetical protein -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 82639.99 Da        Isoelectric Point: 4.3944

>NTDB_id=1012162 H1W81_RS04975 WP_011226133.1 958658..960868(+) (recJ) [Streptococcus thermophilus isolate STH_CIRM_67]
MITSTYDWKINTKEPDAGFFKLAKEHGLVKTAAKIAYERGIRTEEALEDFLKADLSHLHDPYLLHDMDKAVARIRQAIEN
YEQILVYGDYDADGMTSASIVKEALEMMGAEARVYLPNRFTDGYGPNESVYKYFIEQEGISLIITVDNGVAGNEAIAYAQ
EQGVDVIVTDHHSMPSQLPEAYAIVHPEHPGADYPFKYLAGCGVAFKIATALLETIPTEMLDLVAIGTIADMVSLTDENR
IMVKVGLEILKTTERIGLQELLRISEVDLTTISEETVAFKLAPQLNALGRLDDPNPAIELLTGFDDEEAQAIALEINAKN
EERKEVVQNIFDEAMSMVDLDKPVQVLAKEGWHPGVLGIVAGRIMEQISQTVVVLNIEDGLARGSARSLESINIFHALDD
HRDIFTAFGGHAGAAGMTLPEENLTQLSDILCSYVYDNDIDTGAKNTLNLDEELQLGELSLDTIRSLEKLAPFGMDNKKP
VFWLHDITVTQARTMGQNGAHLKFKVKQGKASFDVVAFNKGHLLQEFQQAQGLELAVTLSVNVWNGQTTLQLMLADVRVD
NVQLFDFRSKNMVLPEGLPTVEEASDEATAVVLDTLPNSETELKEWFDGKEFQAIYFKNRIKEAYYLTGYGTREQFARLY
KTIYQFPEFDVRYKLDDLSHYLKIDKILLIKMIQIFDELGFVTIDNGVMTVNKEAGKRDIEESKIFQDLKRLVKFQELMA
LGTPQEIYDWLYNQEQ

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=1012162 H1W81_RS04975 WP_011226133.1 958658..960868(+) (recJ) [Streptococcus thermophilus isolate STH_CIRM_67]
ATGATTACATCAACGTATGATTGGAAAATAAACACAAAAGAGCCAGATGCTGGCTTTTTTAAACTTGCTAAAGAACATGG
ACTGGTAAAAACAGCAGCCAAGATTGCCTATGAACGTGGCATTAGGACAGAAGAGGCACTTGAAGACTTTCTAAAGGCAG
ATTTATCGCACCTTCATGATCCTTATCTGCTTCATGATATGGATAAGGCAGTGGCTAGAATCCGTCAGGCTATTGAAAAT
TATGAACAAATCTTAGTATATGGAGATTACGATGCGGATGGTATGACGTCAGCCTCGATTGTCAAAGAAGCCCTTGAAAT
GATGGGAGCTGAAGCTCGAGTATACTTGCCAAACCGTTTTACAGATGGTTATGGACCAAACGAAAGTGTCTATAAGTATT
TCATTGAGCAGGAAGGTATTTCACTCATCATTACAGTGGATAATGGGGTAGCTGGGAATGAGGCTATCGCTTATGCGCAA
GAGCAAGGTGTGGATGTTATCGTCACAGACCACCATAGCATGCCGTCACAGCTTCCAGAAGCTTATGCCATTGTTCATCC
AGAACATCCAGGCGCTGATTATCCCTTTAAGTATTTGGCAGGTTGTGGAGTAGCTTTCAAAATAGCAACGGCCCTCCTCG
AGACCATACCAACAGAGATGTTGGATTTGGTAGCTATAGGGACTATTGCTGATATGGTTAGCCTGACCGATGAAAATCGT
ATTATGGTTAAGGTTGGACTTGAAATTCTCAAGACTACTGAGCGTATTGGGCTTCAAGAATTGCTCCGTATTTCCGAAGT
AGACCTAACAACAATTTCTGAAGAAACAGTGGCTTTCAAACTAGCCCCTCAACTCAATGCCTTAGGACGTTTAGACGATC
CAAACCCAGCTATTGAACTATTAACCGGATTTGACGATGAGGAAGCGCAAGCCATTGCTCTGGAAATCAATGCCAAAAAT
GAAGAACGTAAGGAAGTTGTTCAAAACATCTTTGATGAAGCCATGAGCATGGTAGACTTGGATAAACCAGTTCAGGTTTT
GGCTAAGGAAGGCTGGCATCCTGGTGTACTCGGCATTGTTGCTGGCCGCATCATGGAGCAAATCAGTCAGACAGTAGTGG
TTTTAAATATTGAAGATGGCTTGGCTAGGGGGTCAGCTCGTAGTTTGGAATCTATCAATATCTTCCATGCACTTGATGAC
CACAGAGACATCTTTACTGCATTTGGAGGGCACGCTGGAGCAGCTGGTATGACCCTTCCAGAGGAAAATCTTACTCAACT
CTCAGATATTTTATGTAGTTATGTCTACGATAATGACATTGATACGGGTGCCAAAAATACGCTTAATTTAGACGAGGAGC
TACAACTCGGTGAGCTTAGCTTGGATACCATAAGGAGCCTCGAAAAACTAGCACCTTTTGGTATGGATAATAAAAAGCCA
GTCTTTTGGCTACATGATATTACCGTTACTCAAGCTAGAACTATGGGGCAAAATGGAGCTCATCTTAAGTTTAAGGTTAA
GCAAGGCAAGGCTAGTTTTGATGTTGTCGCCTTTAACAAAGGGCATCTGCTTCAAGAATTTCAGCAAGCTCAGGGGTTGG
AATTAGCAGTGACGCTGTCCGTTAATGTATGGAATGGCCAGACAACACTACAGCTCATGTTGGCAGATGTGCGCGTGGAC
AATGTTCAGTTATTTGATTTTCGCTCAAAAAATATGGTACTACCTGAGGGCCTACCAACTGTAGAAGAGGCCTCAGACGA
AGCAACTGCAGTTGTCCTCGACACCCTACCGAATTCAGAGACGGAATTAAAAGAGTGGTTTGACGGTAAGGAATTCCAGG
CCATTTATTTTAAGAATCGCATTAAGGAAGCCTATTATCTGACAGGCTATGGAACTAGAGAGCAATTTGCAAGGCTCTAT
AAAACCATTTATCAATTCCCAGAATTTGATGTGCGCTATAAATTAGATGACCTCAGTCATTATCTTAAGATTGATAAAAT
TCTTCTCATCAAGATGATTCAAATTTTTGATGAATTGGGCTTTGTCACTATTGATAATGGTGTCATGACAGTTAATAAAG
AGGCTGGAAAACGTGATATTGAGGAAAGCAAGATTTTCCAGGACCTTAAACGTCTTGTCAAGTTCCAAGAACTCATGGCT
TTGGGAACACCACAGGAAATTTATGATTGGCTCTACAATCAGGAGCAGTAG

Domains

Predicted by InterProScan.

RecJ OB domain (RecJ_OB)

(451-556)

DHHA1 domain (DHHA1)

(317-431)

DHH family (DHH)

(83-228)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(583-731)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M3Y5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

67.027

100

0.674
  recJ Bacillus subtilis subsp. subtilis str. 168

37.26

100

0.395

Multiple sequence alignment