Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AADG64_RS00755 Genome accession   NZ_CP158973
Coordinates   169062..170507 (+) Length   481 a.a.
NCBI ID   WP_024067399.1    Uniprot ID   A0AAX1D9P1
Organism   Achromobacter xylosoxidans strain zafu-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 164062..175507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADG64_RS00740 (AADG64_00740) leuA 165143..166852 (-) 1710 WP_054482354.1 2-isopropylmalate synthase -
  AADG64_RS00745 (AADG64_00745) - 167243..168220 (-) 978 WP_006386985.1 tripartite tricarboxylate transporter substrate binding protein -
  AADG64_RS00750 (AADG64_00750) - 168413..169063 (-) 651 WP_026384934.1 hypothetical protein -
  AADG64_RS00755 (AADG64_00755) rpoN/rpoN1 169062..170507 (+) 1446 WP_024067399.1 RNA polymerase factor sigma-54 Machinery gene
  AADG64_RS00760 (AADG64_00760) - 170601..170807 (+) 207 WP_024067400.1 hypothetical protein -
  AADG64_RS00765 (AADG64_00765) - 170877..171155 (+) 279 WP_006386989.1 type II toxin-antitoxin system RelE/ParE family toxin -
  AADG64_RS00770 (AADG64_00770) - 171334..171633 (+) 300 WP_006386990.1 (2Fe-2S)-binding protein -
  AADG64_RS00775 (AADG64_00775) - 171603..172523 (-) 921 WP_006386991.1 LysR substrate-binding domain-containing protein -
  AADG64_RS00780 (AADG64_00780) - 172688..173551 (+) 864 WP_227740234.1 DMT family transporter -
  AADG64_RS00785 (AADG64_00785) bfr 173976..174452 (+) 477 WP_006386993.1 bacterioferritin -
  AADG64_RS00790 (AADG64_00790) - 174537..175235 (-) 699 WP_026384932.1 hypothetical protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52482.88 Da        Isoelectric Point: 4.8651

>NTDB_id=1012111 AADG64_RS00755 WP_024067399.1 169062..170507(+) (rpoN/rpoN1) [Achromobacter xylosoxidans strain zafu-3]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLELEAEISQVLADNPLLERDDEPASVETQAESERESSVQDDEP
APERDTPVEEMPGSGGVYPDDDSSLPEAARPDTLREHLLGQLVLTRAAPRDAALAALLIDELDENGYLGSPLEEILGWLP
AEMEPDLDELRAALSLLQSFDPAGIGARDMSECLLLQLRSPDTARLPEAADPKALACARLVCAQHLALLATGNHARLCAA
VGCDEATLRAAHALILRLEPRPGRAWTVPAADYAVPDVIVRKTRRGWQVTLNSAAVPRLQINGLYAQMLANQKESAHAGL
QSQLQQAKWMIRNVEQRFDTILRVAQAIVDRQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGAGVSTDGGDATSATAVQTFIRQMVAEENRAKPLSDSQIMEKLADQGIVIARRTVAKYREALRIAPAALRKAQAAA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=1012111 AADG64_RS00755 WP_024067399.1 169062..170507(+) (rpoN/rpoN1) [Achromobacter xylosoxidans strain zafu-3]
ATGCAAAATATTGTACCTATGACCCGTCCTATTTTAGAACTGCGGCCCGGCCAGCATCTGACGCTGACCCCTCAACTGCA
GCAATCGATCCGGTTGCTGCAGCTGTCCACGCTCGAGCTCGAGGCGGAAATCTCGCAAGTGCTCGCCGACAATCCGTTGC
TGGAACGCGACGATGAGCCTGCCTCCGTCGAGACGCAGGCCGAATCCGAGCGCGAGTCGTCGGTGCAGGACGACGAACCC
GCGCCCGAGCGCGACACCCCGGTCGAGGAAATGCCGGGTTCGGGCGGGGTCTACCCCGATGATGATTCCAGCCTGCCCGA
GGCCGCCCGGCCCGATACCCTGCGCGAGCACCTGCTCGGGCAACTGGTGCTGACCCGCGCCGCGCCGCGCGACGCCGCGC
TGGCGGCGCTGCTGATCGACGAACTGGACGAGAACGGCTACCTGGGCTCGCCCCTGGAGGAAATCCTGGGCTGGCTGCCG
GCCGAGATGGAGCCGGACCTGGACGAGCTGCGCGCCGCGCTGTCGCTGCTGCAATCGTTCGACCCGGCCGGCATCGGCGC
GCGCGACATGTCCGAGTGCCTGCTGTTGCAATTGCGCAGTCCCGACACGGCCCGGTTGCCCGAGGCCGCGGATCCCAAGG
CGCTGGCCTGCGCCCGCCTGGTCTGCGCCCAGCATCTGGCGCTGTTGGCCACCGGCAACCATGCCAGGCTGTGCGCCGCG
GTCGGTTGTGACGAGGCCACGCTGCGCGCGGCGCACGCCCTGATCCTGCGTCTGGAGCCCCGTCCGGGCCGCGCCTGGAC
GGTCCCGGCGGCCGATTACGCCGTGCCCGACGTCATCGTGCGCAAGACGCGCCGCGGCTGGCAGGTGACGCTCAACAGCG
CCGCCGTGCCGCGACTGCAGATCAATGGCCTGTACGCCCAAATGCTGGCCAACCAGAAGGAGTCGGCCCACGCCGGCTTG
CAGTCTCAGCTGCAACAGGCGAAATGGATGATCCGCAACGTGGAGCAGCGCTTCGACACCATCCTGCGAGTGGCCCAGGC
CATTGTCGACCGCCAGACCGCCTTCTTCAGCCAGGGGCCGGCGGCCATGCGCCCGCTCATCCTCAAGGACATTGCCGGCG
AGCTGGGCCTGCATGAGTCCACCATTTCACGCGCCACGACACAGAAGTACATGCTCACTCCCTTCGGTACGCTGGAGCTC
AAGCGCTTCTTCGGGGCGGGGGTGTCGACCGATGGCGGCGACGCCACATCCGCCACGGCGGTGCAGACTTTCATCCGCCA
GATGGTGGCCGAGGAGAACCGGGCCAAGCCGCTGTCCGACAGCCAGATCATGGAAAAACTGGCCGATCAGGGCATCGTCA
TCGCCCGCAGGACGGTCGCGAAATACCGCGAGGCACTGCGGATCGCGCCGGCCGCGCTGCGCAAGGCGCAGGCCGCGGCG
CGGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.505

100

0.52

Multiple sequence alignment