Detailed information NTG1012017

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ABUH88_RS20990
• Genome accession: NZ_CP158677
• Coordinates: 4302392..4303192 (+)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain KPEH168
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4297392..4308192

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABUH88_RS20955 (ABUH88_20975)	gspH	4297548..4298063 (+)	516	WP_032414529.1	type II secretion system minor pseudopilin GspH	-
ABUH88_RS20960 (ABUH88_20980)	gspI	4298060..4298425 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
ABUH88_RS20965 (ABUH88_20985)	gspJ	4298422..4299018 (+)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
ABUH88_RS20970 (ABUH88_20990)	gspK	4299015..4300001 (+)	987	WP_077254116.1	type II secretion system minor pseudopilin GspK	-
ABUH88_RS20975 (ABUH88_20995)	gspL	4299988..4301181 (+)	1194	WP_032434208.1	type II secretion system protein GspL	-
ABUH88_RS20980 (ABUH88_21000)	-	4301174..4301659 (+)	486	WP_004145896.1	type II secretion system protein M	-
ABUH88_RS20985 (ABUH88_21005)	-	4301649..4302395 (+)	747	WP_032434210.1	type II secretion system protein N	-
ABUH88_RS20990 (ABUH88_21010)	pilD	4302392..4303192 (+)	801	WP_004213441.1	prepilin peptidase	Machinery gene
ABUH88_RS20995 (ABUH88_21015)	hrpB	4303240..4305669 (-)	2430	WP_032434212.1	ATP-dependent helicase HrpB	-
ABUH88_RS21000 (ABUH88_21020)	thpR	4305742..4306278 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
ABUH88_RS21005 (ABUH88_21025)	sfsA	4306278..4306994 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
ABUH88_RS21010 (ABUH88_21030)	dksA	4307157..4307612 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=1012017 ABUH88_RS20990 WP_004213441.1 4302392..4303192(+) (pilD) [Klebsiella pneumoniae strain KPEH168]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=1012017 ABUH88_RS20990 WP_004213441.1 4302392..4303192(+) (pilD) [Klebsiella pneumoniae strain KPEH168]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425