Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LYX99_RS19460 Genome accession   NZ_CP158565
Coordinates   526727..528232 (-) Length   501 a.a.
NCBI ID   WP_027789104.1    Uniprot ID   A0A104AWL9
Organism   Burkholderia cepacia strain JK11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 521727..533232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYX99_RS19435 (LYX99_019440) - 522754..523389 (-) 636 WP_021162603.1 LON peptidase substrate-binding domain-containing protein -
  LYX99_RS19440 (LYX99_019445) rapZ 523417..524325 (-) 909 WP_021162602.1 RNase adapter RapZ -
  LYX99_RS19445 (LYX99_019450) hprK 524401..525369 (-) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  LYX99_RS19450 (LYX99_019455) - 525501..526040 (-) 540 WP_034195946.1 PTS sugar transporter subunit IIA -
  LYX99_RS19455 (LYX99_019460) hpf 526209..526568 (-) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LYX99_RS19460 (LYX99_019465) rpoN/rpoN1 526727..528232 (-) 1506 WP_027789104.1 RNA polymerase factor sigma-54 Machinery gene
  LYX99_RS19465 (LYX99_019470) lptB 528404..529180 (-) 777 WP_021162599.1 LPS export ABC transporter ATP-binding protein -
  LYX99_RS19470 (LYX99_019475) lptA 529177..529842 (-) 666 WP_021162598.1 lipopolysaccharide transport periplasmic protein LptA -
  LYX99_RS19475 (LYX99_019480) lptC 529863..530477 (-) 615 WP_059688689.1 LPS export ABC transporter periplasmic protein LptC -
  LYX99_RS19480 (LYX99_019485) - 530481..531017 (-) 537 WP_021162596.1 HAD family hydrolase -
  LYX99_RS19485 (LYX99_019490) kdsD 531017..532000 (-) 984 WP_021162595.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54797.94 Da        Isoelectric Point: 4.8767

>NTDB_id=1011733 LYX99_RS19460 WP_027789104.1 526727..528232(-) (rpoN/rpoN1) [Burkholderia cepacia strain JK11]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNSSSSSSERAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1011733 LYX99_RS19460 WP_027789104.1 526727..528232(-) (rpoN/rpoN1) [Burkholderia cepacia strain JK11]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TAGCGAGCCCGCTGCGTGTCGCAGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGCCCAGAACACCGAGCCCGCTGCG
ACAAACAGTAGCAGTTCGTCGAGCGAGCGCGCCGAGCGCGACGAACCGGCCGGCGCGGACGAATACGACGGTTACGCGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATTTGGCCGCGCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGTTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGTGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCACTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGTCGGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCAATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A104AWL9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.047

100

0.689

Multiple sequence alignment