Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ABS324_RS10985 Genome accession   NZ_CP158496
Coordinates   2099973..2100407 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain NBNZ-2162     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2094973..2105407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABS324_RS10965 (ABS324_10965) - 2095887..2097050 (-) 1164 WP_000434574.1 aminotransferase A -
  ABS324_RS10970 (ABS324_10970) - 2097173..2097373 (-) 201 WP_000929276.1 hypothetical protein -
  ABS324_RS10975 (ABS324_10975) kinB 2097684..2098958 (+) 1275 WP_016118571.1 sporulation sensor histidine kinase KinB -
  ABS324_RS10980 (ABS324_10980) - 2098997..2099746 (-) 750 WP_000388471.1 DUF3967 domain-containing protein -
  ABS324_RS10985 (ABS324_10985) nucA/comI 2099973..2100407 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  ABS324_RS10990 (ABS324_10990) comJ 2100444..2100833 (+) 390 WP_000424030.1 competence protein ComJ -
  ABS324_RS10995 (ABS324_10995) metE 2100872..2103160 (-) 2289 WP_001007614.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  ABS324_RS11000 (ABS324_11000) - 2103673..2104230 (-) 558 WP_000062063.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=1011612 ABS324_RS10985 WP_000811494.1 2099973..2100407(+) (nucA/comI) [Bacillus cereus strain NBNZ-2162]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1011612 ABS324_RS10985 WP_000811494.1 2099973..2100407(+) (nucA/comI) [Bacillus cereus strain NBNZ-2162]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTGGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACAAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACCGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment