Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ABS324_RS03200 Genome accession   NZ_CP158496
Coordinates   627103..628479 (-) Length   458 a.a.
NCBI ID   WP_001085202.1    Uniprot ID   -
Organism   Bacillus cereus strain NBNZ-2162     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 622103..633479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABS324_RS03180 (ABS324_03180) ispF 623466..623942 (-) 477 WP_000488388.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ABS324_RS03185 (ABS324_03185) ispD 624059..624739 (-) 681 WP_000288286.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ABS324_RS03190 (ABS324_03190) - 624756..625865 (-) 1110 WP_000919677.1 PIN/TRAM domain-containing protein -
  ABS324_RS03195 (ABS324_03195) disA 626026..627099 (-) 1074 WP_000392159.1 DNA integrity scanning diadenylate cyclase DisA -
  ABS324_RS03200 (ABS324_03200) radA 627103..628479 (-) 1377 WP_001085202.1 DNA repair protein RadA Machinery gene
  ABS324_RS03205 (ABS324_03205) clpC 628575..631010 (-) 2436 WP_000971184.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  ABS324_RS03210 (ABS324_03210) - 631033..632097 (-) 1065 WP_000050843.1 protein arginine kinase -
  ABS324_RS03215 (ABS324_03215) - 632102..632650 (-) 549 WP_098617322.1 UvrB/UvrC motif-containing protein -
  ABS324_RS03220 (ABS324_03220) ctsR 632821..633282 (-) 462 WP_001244558.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49844.48 Da        Isoelectric Point: 7.1422

>NTDB_id=1011588 ABS324_RS03200 WP_001085202.1 627103..628479(-) (radA) [Bacillus cereus strain NBNZ-2162]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNSLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADASYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=1011588 ABS324_RS03200 WP_001085202.1 627103..628479(-) (radA) [Bacillus cereus strain NBNZ-2162]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAATCACCAAAATATATGGGTAAATGTCCAGGGTG
TGGTCAATGGAATTCGCTTGTTGAAGAGATGGAACCCGTTGTATCATCCAGACGCCTAAATTATGCAAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTTACAGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACAAAATTTCAGGAGTTT
AACCGTGTACTAGGTGGCGGAATTGTAGATGGATCTTTAGTACTTATTGGTGGGGACCCTGGGATTGGAAAATCAACGTT
ATTATTACAGATTTCCTCGCAATTAGCAGATGCTTCATATGATGTATTGTATATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACAGATTTACAGCGTATTGCA
GCACATATTGAAGAGATGAATCCTGCTTTTGTTGTTATTGATTCTATTCAAACAATACATTTACCTGAAGTGACATCAGC
TCCTGGTAGTGTGGCGCAAGTACGTGAATGTACAGCAGAATTAATGAAACTTGCAAAAACAAAGGGAATCCCAATTTTTA
TCGTAGGGCATGTGACAAAAGAAGGTGCAATTGCGGGACCGCGTATGCTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACATATCGTATTTTACGAGCTGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATCTT
TGAAATGAAAGAACTTGGCCTTGCAGAAGTCTTAAATCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGAGTTGCAG
GTTCAACAGTAGTTGCCTCGATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAGGCATTAATTTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACAGGAATTGATCATAATCGTGTCTCGCTTATTATGGCGGTACTAGAGAAAAGAACAGG
TTTACTATTACAAAATCAAGATGCGTATTTAAAAGTAGCTGGTGGTTTGAAATTAGATGAACCGGCAATTGATTTAGCTG
TTGCCTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACTGATGCGGTAATAGGAGAAGTTGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATCATTCC
TAGAAAAAATTTAGGGGGGTGGACAATTCCAGAGGGGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631

Multiple sequence alignment