Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ABOE65_RS01805 Genome accession   NZ_CP158358
Coordinates   335033..335470 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain HY23     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 330033..340470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABOE65_RS01780 (ABOE65_01775) - 331052..332188 (+) 1137 WP_012116766.1 zinc-dependent alcohol dehydrogenase -
  ABOE65_RS01785 (ABOE65_01780) - 332203..332637 (+) 435 WP_012116767.1 RDD family protein -
  ABOE65_RS01790 (ABOE65_01785) - 332710..333033 (+) 324 WP_007409357.1 YckD family protein -
  ABOE65_RS01795 (ABOE65_01790) - 333137..334573 (+) 1437 WP_012116768.1 family 1 glycosylhydrolase -
  ABOE65_RS01800 (ABOE65_01795) nin/comJ 334614..335012 (-) 399 WP_007609299.1 competence protein ComJ Regulator
  ABOE65_RS01805 (ABOE65_01800) nucA/comI 335033..335470 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ABOE65_RS01810 (ABOE65_01805) hxlB 335829..336386 (-) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  ABOE65_RS01815 (ABOE65_01810) hxlA 336383..337018 (-) 636 WP_012116769.1 3-hexulose-6-phosphate synthase -
  ABOE65_RS01820 (ABOE65_01815) - 337250..337612 (+) 363 WP_012116770.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1010829 ABOE65_RS01805 WP_003156588.1 335033..335470(-) (nucA/comI) [Bacillus velezensis strain HY23]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1010829 ABOE65_RS01805 WP_003156588.1 335033..335470(-) (nucA/comI) [Bacillus velezensis strain HY23]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment