Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BIKONL_RS09695 Genome accession   NZ_CP158159
Coordinates   2148101..2149471 (+) Length   456 a.a.
NCBI ID   WP_003251626.1    Uniprot ID   I7AVL1
Organism   Pseudomonas putida strain CRA-04     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2143101..2154471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIKONL_RS09665 (BIKONL_001948) cydB 2143212..2144219 (+) 1008 WP_010955308.1 cytochrome d ubiquinol oxidase subunit II -
  BIKONL_RS09670 (BIKONL_001949) - 2144229..2144384 (+) 156 WP_003251614.1 DUF2474 domain-containing protein -
  BIKONL_RS09675 (BIKONL_001950) - 2144490..2145614 (-) 1125 WP_350298576.1 methyltransferase -
  BIKONL_RS09680 (BIKONL_001951) - 2145711..2146418 (-) 708 WP_350298577.1 autoinducer binding domain-containing protein -
  BIKONL_RS09685 (BIKONL_001952) - 2146634..2147410 (+) 777 WP_003251619.1 ferredoxin--NADP reductase -
  BIKONL_RS09690 (BIKONL_001953) mscL 2147506..2147925 (-) 420 WP_003251622.1 large-conductance mechanosensitive channel protein MscL -
  BIKONL_RS09695 (BIKONL_001954) radA 2148101..2149471 (+) 1371 WP_003251626.1 DNA repair protein RadA Machinery gene
  BIKONL_RS09700 (BIKONL_001955) - 2149507..2151024 (-) 1518 WP_350298578.1 nucleobase:cation symporter-2 family protein -
  BIKONL_RS09705 (BIKONL_001956) - 2151288..2151650 (-) 363 WP_029615590.1 PilZ domain-containing protein -
  BIKONL_RS09710 (BIKONL_001957) - 2151828..2153894 (+) 2067 WP_350298579.1 carbon starvation CstA family protein -
  BIKONL_RS09715 (BIKONL_001958) - 2153912..2154109 (+) 198 WP_003251633.1 YbdD/YjiX family protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 48741.18 Da        Isoelectric Point: 6.8985

>NTDB_id=1009995 BIKONL_RS09695 WP_003251626.1 2148101..2149471(+) (radA) [Pseudomonas putida strain CRA-04]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMIESGGAAAPSSGRAGWTGQQAQIKTLAEVSVEEIPRFTTSSTE
LDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAVGMPALYVTGEESQQQVAMRSRRLGLPQDQLKVMTETCIETII
ATARVEKPRVMVIDSIQTIFTEQLQSAPGGVAQVRESTALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYF
EGESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRTQEEVPGSVVMATWEGTRPMLVEVQALVDDSHL
ANPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAVMSSLRNRPLAHGLLVFGEIGLS
GEVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1009995 BIKONL_RS09695 WP_003251626.1 2148101..2149471(+) (radA) [Pseudomonas putida strain CRA-04]
ATGGCCAAGGCCAAGCGCTTGTATGGCTGCACCGAATGCGGCGCGACCTTCCCCAAATGGGCCGGCCAGTGCGGCGAATG
CGGGGCCTGGAACACCCTGGTCGAAACCATGATCGAAAGCGGCGGCGCTGCTGCACCCAGCAGCGGTCGCGCTGGCTGGA
CAGGGCAGCAGGCGCAGATCAAGACCCTGGCCGAAGTCAGTGTCGAGGAAATCCCGCGTTTCACGACCAGCAGCACCGAA
CTGGACCGCGTGCTGGGTGGCGGCCTGGTGGATGGCTCGGTGGTGCTGATTGGCGGTGACCCCGGCATCGGCAAGTCGAC
CATTTTGCTACAGACCTTGTGCAACATTGCCGTGGGCATGCCGGCCCTGTATGTCACGGGGGAGGAGTCGCAGCAGCAGG
TGGCCATGCGCTCGCGGCGGCTGGGCCTACCCCAGGACCAGCTCAAGGTGATGACCGAAACCTGCATCGAGACGATCATT
GCCACGGCGCGGGTCGAGAAGCCGCGGGTAATGGTGATCGACTCGATCCAGACCATTTTCACCGAGCAGTTGCAATCGGC
CCCGGGCGGCGTGGCCCAGGTGCGCGAAAGCACAGCGTTGCTGGTGCGCTATGCCAAGCAAAGCGGTACGGCAATCTTCC
TGGTCGGCCACGTGACCAAGGAAGGCTCGCTGGCTGGCCCAAGGGTGCTGGAGCACATGGTCGACACGGTGCTGTATTTC
GAAGGTGAGTCCGATGGCCGCCTGCGCTTACTGCGGGCGGTCAAGAACCGTTTTGGCGCGGTCAATGAGCTGGGCGTGTT
CGGCATGACCGATCGTGGCCTCAAGGAGGTATCCAACCCGTCGGCGATCTTCCTCAACCGTACCCAGGAAGAGGTGCCGG
GTAGCGTGGTGATGGCAACCTGGGAGGGTACCCGGCCAATGCTGGTCGAGGTGCAGGCGCTGGTCGACGACAGCCATCTG
GCCAACCCGCGGCGGGTAACCTTGGGCCTGGACCAGAACCGTCTGGCCATGTTGTTGGCGGTACTCCACCGCCATGGCGG
TATCCCGACCCATGACCAGGATGTGTTTCTCAACGTGGTCGGCGGGGTGAAGGTGCTGGAAACGGCTTCGGACCTGGCGC
TGCTGGCGGCGGTGATGTCCAGCTTGCGCAACCGGCCACTGGCCCATGGTTTGCTGGTGTTTGGCGAAATCGGCTTGTCG
GGCGAGGTACGGCCGGTGCCCAGTGGCCAGGAGCGCTTGAAGGAAGCGGCCAAGCACGGCTTCAAGCGTGCCATCGTGCC
CAAGGGCAATGCGCCGAAAGAAGCGCCTGCGGGGTTGCAGGTGATTGCCGTTACCCGGCTGGAACAGGCGCTGGATGCAT
TGTTCGAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(355-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I7AVL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

100

0.487
  radA Streptococcus pneumoniae Rx1

46.087

100

0.465
  radA Streptococcus pneumoniae D39

46.087

100

0.465
  radA Streptococcus pneumoniae TIGR4

46.087

100

0.465
  radA Streptococcus pneumoniae R6

46.087

100

0.465
  radA Streptococcus mitis NCTC 12261

45.87

100

0.463
  radA Streptococcus mitis SK321

46.053

100

0.461

Multiple sequence alignment