Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABJ384_RS12475 Genome accession   NZ_CP157981
Coordinates   2577614..2578375 (-) Length   253 a.a.
NCBI ID   WP_349927675.1    Uniprot ID   A0AAU7SVP9
Organism   Acinetobacter sp. A1-4-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2572614..2583375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABJ384_RS12455 (ABJ384_12445) - 2573656..2574357 (-) 702 WP_166169254.1 tetratricopeptide repeat protein -
  ABJ384_RS12460 (ABJ384_12450) hisS 2574387..2575682 (-) 1296 WP_349927672.1 histidine--tRNA ligase -
  ABJ384_RS12465 (ABJ384_12455) ispG 2575686..2576807 (-) 1122 WP_086195369.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABJ384_RS12470 (ABJ384_12460) - 2576832..2577623 (-) 792 WP_349927673.1 helix-turn-helix domain-containing protein -
  ABJ384_RS12475 (ABJ384_12465) pilF 2577614..2578375 (-) 762 WP_349927675.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABJ384_RS12480 (ABJ384_12470) rlmN 2578436..2579671 (-) 1236 WP_086195366.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABJ384_RS12485 (ABJ384_12475) ndk 2579794..2580225 (-) 432 WP_086195365.1 nucleoside-diphosphate kinase -
  ABJ384_RS12490 (ABJ384_12480) iscX 2580337..2580537 (-) 201 WP_092819025.1 Fe-S cluster assembly protein IscX -
  ABJ384_RS12495 (ABJ384_12485) - 2580627..2581178 (-) 552 WP_349927678.1 phosphatase PAP2 family protein -
  ABJ384_RS12500 (ABJ384_12490) - 2581210..2582490 (-) 1281 WP_349927680.1 glycosyltransferase family 1 protein -
  ABJ384_RS12505 (ABJ384_12495) - 2582694..2583107 (-) 414 WP_349927682.1 hypothetical protein -

Sequence

Protein


Download         Length: 253 a.a.        Molecular weight: 28602.46 Da        Isoelectric Point: 9.2227

>NTDB_id=1009485 ABJ384_RS12475 WP_349927675.1 2577614..2578375(-) (pilF) [Acinetobacter sp. A1-4-2]
MSAIFVAGCQTPATIGKKDPEKAVKVRTQLAAEYIKSGDLDSAKRTLDQALEVDSRDSTANMMMGVLLQQEGSKISMDKA
ESYFKRAISADPKNAQARNNYGTYLYQIERYNDALEQLNVAGTMLGYEQRYRALENAGRIYLKQGDMVNAEKTFKQALQV
NRDSYVSMLELSEIFYLKQQTAAATQLYEQFVRSVGQKNQGARALWIGIRIARANSDPMGTQVLVNQLRALFPESPEYQR
YLQLQYSTEAVWK

Nucleotide


Download         Length: 762 bp        

>NTDB_id=1009485 ABJ384_RS12475 WP_349927675.1 2577614..2578375(-) (pilF) [Acinetobacter sp. A1-4-2]
ATGTCCGCAATTTTTGTTGCCGGGTGTCAGACACCAGCAACAATCGGGAAAAAAGATCCTGAGAAGGCAGTGAAAGTTCG
CACTCAATTGGCGGCAGAATATATTAAGTCGGGTGACCTGGATTCAGCAAAACGTACTCTGGATCAGGCACTGGAAGTAG
ATTCACGTGATTCAACAGCAAATATGATGATGGGGGTTTTACTGCAACAAGAGGGCAGTAAAATCAGCATGGACAAAGCA
GAGAGCTATTTTAAGCGCGCAATTTCTGCTGATCCGAAAAATGCACAAGCGCGTAACAACTACGGTACTTATTTGTATCA
AATTGAGCGTTATAATGACGCGCTTGAACAACTCAATGTGGCCGGAACTATGTTGGGCTATGAACAACGCTACAGAGCAT
TGGAAAATGCGGGACGGATTTATTTAAAGCAAGGCGATATGGTGAATGCTGAAAAAACATTCAAACAGGCTCTGCAAGTA
AATCGCGACTCTTATGTTTCAATGTTAGAGTTGTCAGAAATTTTTTATCTCAAACAGCAGACCGCTGCTGCAACACAATT
GTATGAACAGTTTGTGCGCAGTGTTGGTCAAAAGAATCAAGGTGCGCGTGCGCTTTGGATTGGTATCCGAATTGCGCGGG
CAAATAGCGACCCAATGGGAACACAGGTGCTTGTCAATCAGTTGCGCGCGCTTTTTCCCGAGAGTCCGGAATACCAACGT
TATCTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(77-134)

Tetratricopeptide repeat (TPR_8)

(26-55)

Tetratricopeptide repeat (TPR_16)

(136-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

80.08

99.209

0.794
  pilF Acinetobacter baylyi ADP1

71.542

100

0.715

Multiple sequence alignment