Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABNN95_RS30230 Genome accession   NZ_CP157953
Coordinates   3412294..3413799 (-) Length   501 a.a.
NCBI ID   WP_039346023.1    Uniprot ID   A0A1E3FHP0
Organism   Burkholderia contaminans strain UnB1430     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3407294..3418799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNN95_RS30205 (ABNN95_30185) - 3408323..3408958 (-) 636 WP_039346030.1 LON peptidase substrate-binding domain-containing protein -
  ABNN95_RS30210 (ABNN95_30190) rapZ 3408986..3409894 (-) 909 WP_039346027.1 RNase adapter RapZ -
  ABNN95_RS30215 (ABNN95_30195) hprK 3409970..3410938 (-) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  ABNN95_RS30220 (ABNN95_30200) - 3411068..3411607 (-) 540 WP_011353226.1 PTS sugar transporter subunit IIA -
  ABNN95_RS30225 (ABNN95_30205) raiA 3411776..3412135 (-) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ABNN95_RS30230 (ABNN95_30210) rpoN/rpoN1 3412294..3413799 (-) 1506 WP_039346023.1 RNA polymerase factor sigma-54 Machinery gene
  ABNN95_RS30235 (ABNN95_30215) lptB 3413959..3414735 (-) 777 WP_039346017.1 LPS export ABC transporter ATP-binding protein -
  ABNN95_RS30240 (ABNN95_30220) lptA 3414732..3415397 (-) 666 WP_039346006.1 lipopolysaccharide transport periplasmic protein LptA -
  ABNN95_RS30245 (ABNN95_30225) lptC 3415418..3416032 (-) 615 WP_039346004.1 LPS export ABC transporter periplasmic protein LptC -
  ABNN95_RS30250 (ABNN95_30230) - 3416036..3416572 (-) 537 WP_039346001.1 HAD family hydrolase -
  ABNN95_RS30255 (ABNN95_30235) kdsD 3416572..3417555 (-) 984 WP_039345999.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54755.86 Da        Isoelectric Point: 4.8740

>NTDB_id=1009358 ABNN95_RS30230 WP_039346023.1 3412294..3413799(-) (rpoN/rpoN1) [Burkholderia contaminans strain UnB1430]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNGSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1009358 ABNN95_RS30230 WP_039346023.1 3412294..3413799(-) (rpoN/rpoN1) [Burkholderia contaminans strain UnB1430]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCCGCGGACGGATCGCTGATCGCGCAGACGCCTCCCGCGCAGAACACCGAGCCCACTGCG
ACAAACGGTAGCAGTTCGTCGAGCGATCGTGCCGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGATGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGGGTCGGTGCCCGTTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGTTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTTTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCTTCGTCGACAGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCTAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E3FHP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691

Multiple sequence alignment