Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ABM478_RS20305 Genome accession   NZ_CP157848
Coordinates   3996744..3997178 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain B9     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3991744..4002178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABM478_RS20290 - 3992920..3993477 (+) 558 WP_000062067.1 PadR family transcriptional regulator -
  ABM478_RS20295 metE 3993991..3996279 (+) 2289 WP_001007608.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  ABM478_RS20300 comJ 3996318..3996707 (-) 390 WP_000424030.1 competence protein ComJ -
  ABM478_RS20305 nucA/comI 3996744..3997178 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  ABM478_RS20310 - 3997405..3998154 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  ABM478_RS20315 kinB 3998193..3999467 (-) 1275 WP_000420370.1 sporulation sensor histidine kinase KinB -
  ABM478_RS20320 - 3999778..3999978 (+) 201 WP_000929280.1 hypothetical protein -
  ABM478_RS20325 - 4000101..4001264 (+) 1164 WP_000434578.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=1008483 ABM478_RS20305 WP_000811494.1 3996744..3997178(-) (nucA/comI) [Bacillus cereus strain B9]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1008483 ABM478_RS20305 WP_000811494.1 3996744..3997178(-) (nucA/comI) [Bacillus cereus strain B9]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGTTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment